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B5525

Bromophenol Blue sodium salt

Synonym(s):

3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt

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5 g
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$45.10
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About This Item

Empirical Formula (Hill Notation):
C19H9Br4NaO5S
CAS Number:
34725-61-6
Molecular Weight:
691.94
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
57653941
EC Number:
252-170-2
Beilstein/REAXYS Number:
6995701
MDL number:
MFCD00013793

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form

powder

Quality Segment

technique(s)

electrophoresis: suitable

solubility

water: soluble

suitability

suitable for nucleic acid electrophoresis

storage temp.

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.
A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Preparation Note

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

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This Item
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Bromophenol Blue sodium salt

Sigma-Aldrich

B5525

Bromophenol Blue sodium salt

Bromophenol Blue sodium salt Molecular Biology, suitable for electrophoresis

Sigma-Aldrich

B8026

Bromophenol Blue sodium salt

Bromophenol Blue solution 0.04 wt. % in H2O

Sigma-Aldrich

318744

Bromophenol Blue solution

Bromophenol Blue titration: suitable

Sigma-Aldrich

B0126

Bromophenol Blue

description

-

description

Molecular Biology, suitable for electrophoresis

description

0.04 wt. % in H2O

description

titration: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

titration: suitable

technique(s)

titration: suitable

suitability

suitable for nucleic acid electrophoresis

suitability

suitable for (Suitable For Use as a Tracking Dye For Electrophoresis in Alkaline and Neutral Buffers)

suitability

-

suitability

-

solubility

water: soluble

solubility

-

solubility

-

solubility

methanol: 10 mg/mL

form

powder

form

powder

form

liquid

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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Questions

  1. Does Sigma have any shelf-life requirements for B5525?

    1 answer

    1. This product is not assigned an expiration or retest date. Products that are robust in nature and extremely stable, in the original unopened container, are not assigned an expiration date or retest schedule. These products will have no dating reported on either the label or the Certificate of Analysis.
      Products that are not assigned an expiration or retest date are covered by a warranty period of 1 year from the date of product shipment.

      Full Product Dating Information may be reviewed at the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/356/113/product-dating-information-06-25-ms.pdf

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