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B6650

X-GlcA

reagent for selection of recombinant bacterial clones

Synonym(s):

5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, X-GlcA, X-glucuronide CHA salt

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10 mg

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$187.00
25 mg
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$308.00
100 mg
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$924.00

About This Item

Empirical Formula (Hill Notation):
C14H13BrClNO7 · C6H13N
CAS Number:
114162-64-0
Molecular Weight:
521.79
NACRES:
NA.85
PubChem Substance ID:
24891926
UNSPSC Code:
12352200
MDL number:
MFCD00058543

$187.00

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grade

Molecular Biology

Quality Level

200

sterility

non-sterile

assay

≥98% (TLC)

form

powder

technique(s)

nucleic acid detection: suitable

solubility

DMF: soluble

suitability

suitable for β-galactosidase test

storage temp.

−20°C

SMILES string

NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34

InChI

1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1

InChI key

JXCKZXHCJOVIAV-CYRSAHDMSA-N

General description

X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Chromogenic substrate for β-glucuronidase (GUS) gene detection.
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.

Biochem/physiol Actions

When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.

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This Item
B9146S9811B2904
X-GlcA reagent for selection of recombinant bacterial clones

Sigma-Aldrich

B6650

X-GlcA

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside ≥98%

Sigma-Aldrich

B9146

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

S-Gal® reagent for selection of recombinant bacterial clones

Sigma-Aldrich

S9811

S-Gal®

Bluo-Gal reagent for selection of recombinant bacterial clones

Sigma-Aldrich

B2904

Bluo-Gal

technique(s)

nucleic acid detection: suitable

technique(s)

-

technique(s)

-

technique(s)

nucleic acid detection: suitable

grade

Molecular Biology

grade

-

grade

Molecular Biology

grade

Molecular Biology

suitability

suitable for β-galactosidase test

suitability

suitable for identification of lac+ bacterial colonies

suitability

suitable for β-galactosidase test

suitability

suitable for substrate for β-galactosidase

form

powder

form

powder

form

powder

form

powder

solubility

DMF: soluble

solubility

DMF: methanol (1:1): soluble 50 mg/mL

solubility

DMF: 50 mg/mL

solubility

DMF: soluble

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN2572
BCCK0770
BCBM9813V
BCBR2021V
BCBW4472

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Global Trade Item Number

SKUGTIN
B6650-100MG04061833435373
B6650-25MG04061833435496
B6650-10MG04061833435441

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