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Monoclonal Anti-Bovine IgG antibody produced in mouse

Name: Monoclonal Anti-Bovine IgG antibody produced in mouse
Brand: SIGMA
Price: 236 USD
Availability: InStock

clone BG-18, ascites fluid

Synonym(s):

Monoclonal Anti-Bovine IgG

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Pack Size	SKU	Availability	Price
0.2 mL		Available to ship TODAYfromMILWAUKEE	$236.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

BG-18, monoclonal

Application:

DB, ELISA (i)

Citations:

$236.00

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Properties

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

BG-18, monoclonal

contains

15 mM sodium azide

species reactivity

bovine (non-reactive to IgM)

should not react with

pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat

technique(s)

dot blot: suitable, indirect ELISA: 1:1000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction. IgG (immunoglobulin G) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical γ or γ light (L) chains of 50 kDa and 25 kDa, respectively.

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG antibody has been used in indirect immunoperoxidase staining.

Monoclonal Anti-Bovine IgG antibody produced in mouse is suitable for ELISA at a working dilution of 1:20,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. Monoclonal Anti-Bovine IgG antibody produced in mouse was used as an isotype control in ChIP assay.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

bovine IgG1 and IgG2

Physical form

Ascites fluid containing 0.1% sodium azide

Preparation Note

For continuous use, store at 2-8°C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

1 of 1

This Item	I5635	I7260	A7554
Sigma-Aldrich B6901 Monoclonal Anti-Bovine IgG antibody produced in mouse	Sigma-Aldrich I5635 Monoclonal Anti-Human IgG2 antibody produced in mouse	Sigma-Aldrich I7260 Monoclonal Anti-Human IgG3 antibody produced in mouse	Sigma-Aldrich A7554 Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form purified immunoglobulin
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity bovine (non-reactive to IgM)	species reactivity -	species reactivity -	species reactivity bovine (non-reactive to IgM)
technique(s) dot blot: suitable, indirect ELISA: 1:1000	technique(s) indirect ELISA: 1:5,000	technique(s) indirect ELISA: 1:10,000	technique(s) direct ELISA: 1:25,000, dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG, western blot (chemiluminescent): 1:100,000
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate alkaline phosphatase conjugate
isotype IgG1	isotype IgG1	isotype IgG1	isotype IgG1

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000519160

0000483590

0000458256

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The B Cell Response to Foot-and-Mouth Disease Virus in Cattle following Sequential Vaccination with Multiple Serotypes.

Clare F J Grant et al.

Journal of virology, 91(9) (2017-02-24)

Foot-and-mouth disease virus (FMDV) is a highly contagious viral disease. Antibodies are pivotal in providing protection against FMDV infection. Serological protection against one FMDV serotype does not confer interserotype protection. However, some historical data have shown that interserotype protection can

Histological and Immunohistochemical Studies on the Furstenberg's Rosette in Cows.

Asti R N, et al.

Kafkas U?niversitesi veteriner faku?ltesi dergisi, 17(2), 520-520 (2011)

Fabrication of a Novel Conductometric Biosensor for Detecting Mycobacterium avium subsp. paratuberculosis Antibodies.

Chika Okafor et al.

Sensors (Basel, Switzerland), 8(9), 6015-6025 (2008-09-26)

Johne's disease (JD) is one of the most costly bacterial diseases in cattle. In the U.S., economic losses from the disease have been estimated to exceed $1,500,000,000 per year, mainly from the effects of reduced milk production. Current diagnostic tests

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Global Trade Item Number

SKU	GTIN
B6901-.2ML	04061837514876

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