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B7401

Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG

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0.5 mL
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$260.00
1 mL
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$391.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162627
Conjugate:
biotin conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB CL
Citations:
24

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biological source

goat

Quality Level

200

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:160,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, western blot (chemiluminescent): 1:300,000 using total cell extract of HeLa cells (5-10 μg/well)

storage temp.

−20°C

target post-translational modification

unmodified

General description

Goat polyclonal anti-Mouse IgG (Fc specific)–Biotin reacts with mouse IgG and mouse IgG, Fc fragment. It shows no reactivity with mouse IgG Fab fragment, human IgG, IgA, IgM, kappa and lambda light chain, bovine IgG and IgM, or horse IgG.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.

Application

Anti-Mouse IgG (Fc specific)−Biotin antibody produced in goat has been used in ELISA and quantitative suspension array technology (qSAT) multiplex antibody assay.
Goat polyclonal anti-Mouse IgG (Fc specific)–Biotin may be used to detect mouse IgG within serum or other biological systems. The antibody may be used as a secondary antibody in immunochemical assays based on biotin:avidin second antibody signal systems wherein mouse IgG is the primary antibody.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
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Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

B7401

Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat

Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

B9904

Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat

Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

B0529

Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat

Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

B7151

Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

direct ELISA: 1:160,000, western blot (chemiluminescent): 1:300,000 using total cell extract of HeLa cells (5-10 μg/well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400

technique(s)

direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells

technique(s)

direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:300,000-1:600,000 using using Hela cells extract

technique(s)

direct ELISA: 1:200,000-1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells

conjugate

biotin conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

biological source

goat

biological source

goat

biological source

goat

biological source

goat

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000541668
0000541674
0000475489
0000475489
0000541674

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Global Trade Item Number

SKUGTIN
B7401-.5ML04061838352682
B7401-1ML04061837514999

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