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B8520

Anti-Mouse IgG (whole molecule)−Biotin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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1 mL

Available to ship TODAYfromMILWAUKEE

$238.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162641
Conjugate:
biotin conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
20

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biological source

rabbit

Quality Level

200

conjugate

biotin conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:400,000-1:800,000

storage temp.

−20°C

target post-translational modification

unmodified

General description

Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum.

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (whole molecule)-Biotin antibody has been used in
  • immunofluorescence
  • immunoblot
  • direct enzyme linked immunosorbent assay (ELISA)
  • serotyping assay
  • immunohistochemistry

Biochem/physiol Actions

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Mouse IgG (whole molecule)−Biotin antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

B8520

Anti-Mouse IgG (whole molecule)−Biotin antibody produced in rabbit

Anti-Mouse IgG (whole molecule)−TRITC antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

T2402

Anti-Mouse IgG (whole molecule)−TRITC antibody produced in rabbit

Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A8917

Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A9044

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

technique(s)

direct ELISA: 1:200,000, western blot: 1:400,000-1:800,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300

technique(s)

direct immunofluorescence: 1:64, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

technique(s)

direct ELISA: 1:20,000, dot blot: 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

technique(s)

direct ELISA: 1:40,000 using using 5 μg/ml of mouse IgG for coating and OPD substrate, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:80,000-160,000

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

biotin conjugate

conjugate

TRITC conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000471598
0000471598
0000457894
0000457894
0000403420

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Global Trade Item Number

SKUGTIN
B8520-1ML04061833442920

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