Key Documents

Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat

Name: Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat
Brand: SIGMA
Price: 229 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Select a Size

Change View

Size/SKU	Availability	Price
0.5 mL	Check Cart for Availability	$229.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162627

Conjugate:

biotin conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB

Citations:

$229.00

Check Cart for Availability

Request a Custom Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

goat

Quality Segment

200

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

concentration

≥2 mg/mL

technique(s)

direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

IgG is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γchains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.

Application

Anti-Mouse IgG (Fc specific)-Biotin antibody has been used in enzyme linked immunosorbent assay (ELISA), dot blot, immunohistology, nuclear labeling, ELISA-inhibition method. immunohistochemistry.

Anti-Mouse IgG (Fc specific)-Biotin antibody produced in goat was used for immunocytochemistry staining to visualize neonatal myosin in chicken muscle sections. It was used for ELISPOT analysis to study IgG levels in mice infected with cholera toxin.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Mouse IgG (Fc specific) antibody allows for the binding of various labels such as avidin or streptavidin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human or rat samples.

Other Notes

Antibody adsorbed with human IgG and rat serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	M3534	A0168	M2650
Sigma-Aldrich B9904 Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat	Sigma-Aldrich M3534 Anti-Mouse IgG (Fc specific) antibody produced in goat	Sigma-Aldrich A0168 Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat	Sigma-Aldrich M2650 Anti-Mouse IgG (Fc specific) antibody produced in goat
description affinity isolated antibody, buffered aqueous solution	description 2.0 mg/mL, affinity isolated antibody	description affinity isolated antibody	description affinity isolated antibody, buffered aqueous solution
technique(s) direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells	technique(s) indirect ELISA: 1:15,000	technique(s) western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well, direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, immunocytochemistry: suitable	technique(s) indirect ELISA: 1:15,000
conjugate biotin conjugate	conjugate unconjugated	conjugate peroxidase conjugate	conjugate unconjugated
biological source goat	biological source goat	biological source goat	biological source goat
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
species reactivity mouse	species reactivity -	species reactivity mouse	species reactivity -
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

Still not finding the right product?

Explore all of our products under Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat

— or —

Try our Product Selector Tool to narrow your options

Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.