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Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat

Name: Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat
Brand: SIGMA
Price: 187.85 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

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Pack Size	SKU	Availability	Price
0.5 mL		Available to ship TODAYfromMILWAUKEE	$221.00 $187.85

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162627

Conjugate:

biotin conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB

Citations:

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Properties

biological source

goat

Quality Level

200

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

concentration

≥2 mg/mL

technique(s)

direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

IgG is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γchains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.

Application

Anti-Mouse IgG (Fc specific)-Biotin antibody has been used in enzyme linked immunosorbent assay (ELISA), dot blot, immunohistology, nuclear labeling, ELISA-inhibition method. immunohistochemistry.

Anti-Mouse IgG (Fc specific)-Biotin antibody produced in goat was used for immunocytochemistry staining to visualize neonatal myosin in chicken muscle sections. It was used for ELISPOT analysis to study IgG levels in mice infected with cholera toxin.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Mouse IgG (Fc specific) antibody allows for the binding of various labels such as avidin or streptavidin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human or rat samples.

Other Notes

Antibody adsorbed with human IgG and rat serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	B0529	B7401	B7151
Sigma-Aldrich B9904 Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat	Sigma-Aldrich B0529 Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat	Sigma-Aldrich B7401 Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat	Sigma-Aldrich B7151 Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat
technique(s) direct ELISA: 1:50,000, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300	technique(s) direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:300,000-1:600,000 using using Hela cells extract	technique(s) direct ELISA: 1:160,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, western blot (chemiluminescent): 1:300,000 using total cell extract of HeLa cells (5-10 μg/well)	technique(s) direct ELISA: 1:200,000-1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells
biological source goat	biological source goat	biological source goat	biological source goat
species reactivity mouse	species reactivity mouse	species reactivity -	species reactivity -
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate biotin conjugate	conjugate biotin conjugate	conjugate biotin conjugate	conjugate biotin conjugate
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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EphA/ephrin A reverse signaling promotes the migration of cortical interneurons from the medial ganglionic eminence.

André Steinecke et al.

Development (Cambridge, England), 141(2), 460-471 (2014-01-02)

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Kate E Broderick et al.

Human vaccines, 7 Suppl, 22-28 (2011-01-26)

Electropermeabilization of mammalian cells is a technique that has been used for the delivery of therapeutics, such as DNA plasmids or DNA vaccines. Typically, delivery via electropermeabilization occurs through injection of the substance into the tissue of interest followed by

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Global Trade Item Number

SKU	GTIN
B9904-.5ML	04061838352842

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