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Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse

Name: Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse
Brand: SIGMA
Price: 598 USD
Availability: InStock

clone VC1.1, ascites fluid, buffered aqueous solution

Synonym(s):

Monoclonal Anti-HNK-1, Anti-CD57, Anti-N-CAM

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0.2 mL	Check Cart for Availability	$598.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164634

Conjugate:

unconjugated

Clone:

VC1.1, monoclonal

Application:

Citations:

$598.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

VC1.1, monoclonal

form

buffered aqueous solution

contains

15 mM sodium azide

species reactivity

mouse, rat, feline, human

technique(s)

western blot: 1:4,000 using cerebral cortex extract

isotype

IgM

UniProt accession no.

Q9P2W7

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... B3GAT1(27087)
mouse ... B3gat1(76898)
rat ... B3gat1(117108)

Description

General description

CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, about 60% of NK active cells and on a subset of T cells.

Monoclonal Anti-HNK-1/N-CAM (CD57) (mouse IgM isotype) is derived from the VC1.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Human natural killer cell -1 (HNK-1), also referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP, is mapped to human chromosome 11q25. The encoded protein belongs to the glucuronyltransferase gene family. Neural cell-adhesion molecule (N-CAM) is expressed on all neuroepithelial cells from the time of neural induction in the early embryo. It is known that the sulfated carbohydrate HNK-1 epitope is carried by several surface molecules including a proportion of N-CAM isoforms.

Immunogen

A homogenate of cat primary visual cortex (area 17)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse has been used:

as primary antibody for western blot analysis
immunocytochemistry
immunocytochemical
immunohistological staining

Biochem/physiol Actions

HNK1 (human natural killer-1) is referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP. It is a member of the glucuronyltransferase gene family. Human natural killer-1 (HNK1) carbohydrate epitope is expressed on various cell adhesion molecules in the nervous system and plays a role in cell-cell and cell-substrate interactions. HNK-1 epitope stabilizes GluR2 on neuronal surface membranes and regulates cell surface stability of GluR2 by modulating the interaction with N-cadherin. It is also plays a role in cell migration and synaptic plasticity. HNK-1 epitope has been associated with the risk of metastasis. It is observed to be highly expressed in melanoma cell lines.

Recognizes the CD57/HNK-1 human myeloid cell associated surface glycoprotein. The epitope recognized is an N-linked carbohydrate which is present in a variety of glycoproteins and in some glycolipids. It is resistant to formalin fixation and paraffin embedding. VC1.1 antibody and the HNK-1 (Leu7) antibody inhibit the binding of each other. The antibody recognizes myelin associated glycoprotein in some species and a high molecular weight chondroitin sulphate proteoglycan.
5th Workshop: code no. NK 67

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C6680	C9672	C8093
Sigma-Aldrich C0678 Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse	Sigma-Aldrich C6680 Anti-HNK-1/N-CAM (CD57) antibody, Mouse monoclonal	Sigma-Aldrich C9672 Monoclonal Anti-Neural Cell Adhesion Molecule antibody produced in mouse	Sigma-Aldrich C8093 Monoclonal Anti-Connexin-43 antibody produced in mouse
description clone VC1.1, ascites fluid, buffered aqueous solution	description clone VC1.1, purified from hybridoma cell culture	description clone NCAM-0B11, ascites fluid	description clone CXN-6, ascites fluid
species reactivity mouse, rat, feline, human	species reactivity mouse, rat, feline, human	species reactivity rat, human	species reactivity rat, hamster, bovine, mouse, human
biological source mouse	biological source mouse	biological source mouse	biological source mouse
antibody form ascites fluid	antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form ascites fluid
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Gene Information human ... B3GAT1(27087) mouse ... B3gat1(76898) rat ... B3gat1(117108)	Gene Information human ... B3GAT1(27087) mouse ... B3gat1(76898) rat ... B3gat1(117108)	Gene Information human ... NCAM1(4684), NCAM2(4685) rat ... Ncam1(24586), Ncam2(288280)	Gene Information human ... GJA1(2697), GJA1P1(2698) mouse ... Gja1(14609) rat ... Gja1(24392)
clone VC1.1, monoclonal	clone VC1.1, monoclonal	clone NCAM-0B11, monoclonal	clone CXN-6, monoclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c