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C1224

Caspase 3 human

≥85% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein

Synonym(s):

Apopain, CPP32, Yama

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Size/SKUAvailabilityPrice
10 μg

Estimated to ship onNovember 04, 2026fromSAINT LOUIS

$711.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
3.4.22.56 (BRENDA, IUBMB)
MDL number:
MFCD01634593
Specific activity:
≥1.0 units/mg protein
Assay:
≥85% (SDS-PAGE)
Recombinant:
expressed in E. coli (C-terminal histidine-tagged)

$711.00

Estimated to ship onNovember 04, 2026Details

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recombinant

expressed in E. coli (C-terminal histidine-tagged)

Quality Segment

200

assay

≥85% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥1.0 units/mg protein

mol wt

~30 kDa

UniProt accession no.

P42574

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CASP3(836)

General description

Research area: Apoptosis

Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag.

Application

Caspase 3 human has been used to digest human r-vimentin. It has also been used in caspase-3 inhibition assay and protease inhibition assay.

This product may also be used in cell-based apoptosis assay.

Biochem/physiol Actions

Caspase 3 is a member of the CED-3 subfamily of caspases and is responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation. Caspase 3 is considered to be an effector caspase, activating pro-caspase 6 and pro-caspase 9 in vitro. Caspase 3 can be activated by caspase 8, caspase 6, and granzyme B.
Member of the CED-3 subfamily of caspases and responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation.

Physical form

Solution in 10% (w/v) glycerol containing 50 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS

Preparation Note

Caspase 3 is synthesized as a 32 kDa proenzyme. The active enzyme is a heterodimer of two large (17 kDa) subunits and two small (12 kDa) subunits.

Other Notes

One unit will cleave 1.0 μmole of N-acetyl-Asp-Glu-Val-Asp-pNA per min at pH 7.4 at 25 °C.

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Caspase 3 human ≥85% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein

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C1224

Caspase 3 human

Anti-Caspase 3 Antibody, active (cleaved) form Chemicon®, from rabbit

Sigma-Aldrich

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Anti-Caspase 3 Antibody, active (cleaved) form

Anti-Caspase 3, Active antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

C8487

Anti-Caspase 3, Active antibody produced in rabbit

Anti-Caspase3 (active form) Antibody, clone 3D9.3 clone 3D9.3, from mouse

Sigma-Aldrich

MAB10753

Anti-Caspase3 (active form) Antibody, clone 3D9.3

description

≥85% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein

description

Chemicon®, from rabbit

description

IgG fraction of antiserum, buffered aqueous solution

description

clone 3D9.3, from mouse

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)
mouse ... Casp3(12367)
rat ... Casp3(25402)

Gene Information

human ... CASP3(836)

assay

≥85% (SDS-PAGE)

assay

-

assay

-

assay

-

specific activity

≥1.0 units/mg protein

specific activity

-

specific activity

-

specific activity

-

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous solution

form

-

recombinant

expressed in E. coli (C-terminal histidine-tagged)

recombinant

-

recombinant

-

recombinant

-

mol wt

~30 kDa

mol wt

-

mol wt

-

mol wt

-

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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