Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

C-12901

Human Mononuclear Cells (hMNC)

Isolated from cord blood, single donor, ultra-pure, 25 million cryopreserved cells

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
1 ea
Check Cart for Availability
$694.00

About This Item

NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
human umbilical cord blood
Morphology:
(mononuclear)

$694.00

Check Cart for Availability
​
Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

human umbilical cord blood

packaging

pkg of 25,000,000 cells

morphology

(mononuclear)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Application

Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.

Biochem/physiol Actions

Cord Blood

Preparation Note

Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Mononuclear Cells. They are routinely characterized by flow cytometry analyzing a series of cellular parameters, e.g. viability, cell size, granularity. provides detailed information on the percentages of the major cellular sub-populations, i.e., the lymphocytes, the monocytes, and granulocytes, for each lot of Mononuclear Cells. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 1 x 106 cells per mlTested Markers: CD31 negative, CD34 negative, CD105 positive, CD146 positive

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
C-14096C-14098C-12907
Human Mononuclear Cells (hMNC) Isolated from cord blood, single donor, ultra-pure, 25 million cryopreserved cells

Sigma-Aldrich

C-12901

Human Mononuclear Cells (hMNC)

Human Mononuclear Cells (hMNC) Isolated from cord blood, single donor, 1 million cells per pellet

Sigma-Aldrich

C-14096

Human Mononuclear Cells (hMNC)

Human Mononuclear Cells (hMNC) Isolated from peripheral blood, single donor, 1 million cells per pellet

Sigma-Aldrich

C-14098

Human Mononuclear Cells (hMNC)

Human Mononuclear Cells (hMNC) Isolated from peripheral blood, single donor, ultra-pure, 25 million cryopreserved cells

Sigma-Aldrich

C-12907

Human Mononuclear Cells (hMNC)

morphology

(mononuclear)

morphology

(mononuclear)

morphology

(mononuclear)

morphology

(mononuclear)

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | mammalian: suitable

biological source

human umbilical cord blood

biological source

human peripheral blood

biological source

human peripheral blood

biological source

human peripheral blood

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

packaging

pkg of 25,000,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 25,000,000 cells

storage temp.

−196°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−196°C

Still not finding the right product?

Explore all of our products under Human Mononuclear Cells (hMNC)

wgk

WGK 1

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

Hematopoietic Stem & Immune Cell Culture

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

Questions

Reviews

No rating value

Active Filters