Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

C2010

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV, lyophilized powder

Synonym(s):

Con A

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
25 mg

Available to ship TODAYfromMILWAUKEE

$40.00
100 mg

Available to ship TODAYfromMILWAUKEE

$98.80
250 mg

Available to ship TODAYfromMILWAUKEE

$205.00
1 g

Available to ship TODAYfromMILWAUKEE

$479.00

About This Item

CAS Number:
11028-71-0
UNSPSC Code:
23151817
EC Number:
234-258-2
NACRES:
NA.32
MDL number:
MFCD00071069
Form:
lyophilized powder

$40.00

Available to ship TODAYDetails

​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

type

Type IV

Quality Level

300

form

lyophilized powder

potency

<64 μg per mL agglutination activity

impurities

Carbohydrate, essentially free, salt, essentially free

storage temp.

−20°C

General description

Concanavalin A (con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.

Application

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
  • as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs)
  • for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4+ T cells for T cell vaccination and for induction of hepatitis
  • for the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assay
  • for the stimulation of spleen in mitogen assays

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Analysis Note

Highly purified by specific affinity chromatography
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
C2272C7642L7647
Concanavalin A from Canavalia ensiformis (Jack bean) Type IV, lyophilized powder

Sigma-Aldrich

C2010

Concanavalin A from Canavalia ensiformis (Jack bean)

Concanavalin A from Canavalia ensiformis (Jack bean) biotin conjugate, Type IV, lyophilized powder

Sigma-Aldrich

C2272

Concanavalin A from Canavalia ensiformis (Jack bean)

Concanavalin A from Canavalia ensiformis (Jack bean) FITC conjugate, Type IV, lyophilized powder

Sigma-Aldrich

C7642

Concanavalin A from Canavalia ensiformis (Jack bean)

Concanavalin A from Canavalia ensiformis (Jack bean) Type VI, lyophilized powder

Sigma-Aldrich

L7647

Concanavalin A from Canavalia ensiformis (Jack bean)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

impurities

Carbohydrate, essentially free, salt, essentially free

impurities

Carbohydrate, essentially free

impurities

Carbohydrate, essentially free

impurities

Carbohydrate, essentially free, salt, essentially free

potency

<64 μg per mL agglutination activity

potency

-

potency

-

potency

≤20 μg per mL agglutination activity

type

Type IV

type

Type IV

type

Type IV

type

Type VI

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

Still not finding the right product?

Explore all of our products under Concanavalin A from Canavalia ensiformis (Jack bean)

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000533188
0000531372
0000531371
0000531371
0000533188

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Product Information Sheet - C2010

Product Information Sheet

Aditi Varthaman et al.
Journal of immunology (Baltimore, Md. : 1950), 184(12), 6585-6591 (2010-05-22)
Administration of attenuated pathogenic T cell clones, a procedure known as T cell vaccination, induces CD8+ T cells specific for peptides derived from the Vbeta-chain of the TCR presented by the MHC class Ib molecule, Qa-1 expressed on the vaccine
Effects of organochlorine contaminants on loggerhead sea turtle immunity: comparison of a correlative field study and in vitro exposure experiments
Keller J M, et al.
Environmental Health Perspectives, 114(1), 70-76 (2005)
Animal Models of Liver Diseases
Animal Models for the Study of Human Disease, 313-339 (2017)
View All Related Papers

Global Trade Item Number

SKUGTIN
C2010-25MG04061833474983
C2010-250MG04061833474976
C2010-1G04061833474969
C2010-100MG04061833474952

Questions

Reviews

No rating value

Active Filters