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C2306

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–Cy3 antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Rabbit IgG Cy3, Cy3 Anti-Rabbit IgG

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Size/SKUAvailabilityPrice
1 mL
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$374.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162784
Conjugate:
CY3 conjugate
Clone:
polyclonal
Application:
IHC (p)
Citations:
131

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biological source

sheep

Quality Segment

200

conjugate

CY3 conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rabbit

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–Cy3 antibody produced in sheep binds to all rabbit Igs and is useful when trying to avoid background staining due to the presence of Fc receptors.
The monomeric structure of immunoglobulin G (IgG) consists of two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity (Fab fragments).

Application

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment-Cy3 antibody produced in sheep may be used for immunohistochemistry at a dilution of 1:100 and for immunocytochemistry.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment antibody allows for the visualization of proteins by fluorescent microscopy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
C2181A9919A3937
Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–Cy3 antibody produced in sheep affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

C2306

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–Cy3 antibody produced in sheep

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Cy3 antibody produced in sheep affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

C2181

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Cy3 antibody produced in sheep

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A9919

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3937

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment−Alkaline Phosphatase antibody produced in goat

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous glycerol solution

biological source

sheep

biological source

sheep

biological source

goat

biological source

goat

conjugate

CY3 conjugate

conjugate

CY3 conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

direct ELISA: 1:45,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot: 1:100,000-1:200,000 using detecting actin in total cell extract of HeLa cells (5-10 μg per well)

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

species reactivity

rabbit

species reactivity

mouse

species reactivity

rabbit

species reactivity

rabbit

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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