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C2780

Chondroitinase AC from Flavobacterium heparinum

lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

Synonym(s):

Chondroitin AC lyase

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Size/SKUAvailabilityPrice
5 units

Available to ship TODAYfromMILWAUKEE

$645.00

About This Item

CAS Number:
9047-57-8
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
4.2.2.5 (BRENDA, IUBMB)
MDL number:
MFCD00130796
Specific activity:
0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

$645.00

Available to ship TODAYDetails

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conjugate

(Glucosaminoglycan)

Quality Segment

form

lyophilized powder

specific activity

0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

composition

Protein, ~15% Lowry

solubility

0.02 M phosphate buffer: soluble (pH 7.0)

foreign activity

Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount

storage temp.

−20°C

Application

Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.
Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides

Biochem/physiol Actions

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

Preparation Note

Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.

Other Notes

Contains potassium phosphate buffer salts and BSA as stabilizer.
One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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This Item
E2039C8058C0954
Chondroitinase AC from Flavobacterium heparinum lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

Sigma-Aldrich

C2780

Chondroitinase AC from Flavobacterium heparinum

Chondroitinase AC from Flavobacterium heparinum recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis

Sigma-Aldrich

E2039

Chondroitinase AC from Flavobacterium heparinum

Chondroitinase B from Flavobacterium heparinum lyophilized powder (with BSA as stabilizer)

Sigma-Aldrich

C8058

Chondroitinase B from Flavobacterium heparinum

Chondroitinase C from Flavobacterium heparinum lyophilized powder, ≥200 units/mg solid

Sigma-Aldrich

C0954

Chondroitinase C from Flavobacterium heparinum

description

lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

description

recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis

description

lyophilized powder (with BSA as stabilizer)

description

lyophilized powder, ≥200 units/mg solid

specific activity

0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

specific activity

≥200 units/mg protein

specific activity

-

specific activity

≥200 units/mg solid

form

lyophilized powder

form

lyophilized solid

form

lyophilized powder (with BSA as stabilizer)

form

lyophilized powder

solubility

0.02 M phosphate buffer: soluble (pH 7.0)

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

foreign activity

Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount

foreign activity

-

foreign activity

-

foreign activity

-

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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