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C2874

CryoStor® cell cryopreservation media

CS10

Synonym(s):

cell freezing medium

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Pack SizeSKUAvailabilityPrice
100 mL
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$775.00

About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

$775.00

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Quality Level

100

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

shipped in

ambient

storage temp.

2-8°C

General description

The CryoStor CS2, CS5, and CS10 family of preservation solutions represents the next generation of cryopreservation media. Designed to prepare and preserve cells in ultra low temperature environments (–80 to –196 °C), CryoStor media provide a safe, protective environment for cells and tissues during thefreezing, storage, and thawing process. Through modulating the cellular biochemical response to the cryopreservation process, these media provide enhanced cell viability and functionality, while eliminating the need to include serum, proteins, or high levels of cytotoxic agents.

CryoStor CS2, CS5, and CS10 are a series of cell specific, optimized preservation media, uniquely formulated to address the molecular biological aspects of cells during the cryopreservation process; thereby, directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.

These media are recommended for the preservation of stem cells, hepatocytes, tissue samples, and other extremely sensitive cell types.

Application

CryoStor, a series of cell-specific, optimized preservation media, is uniquely formulated to address the molecularbiological aspects of cells during the cryopreservation process thereby directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function.
CryoStor® cell cryopreservation media has been used in:
  • the preservation of T cells
  • the preservation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
  • hiPSC-derived cardiac progenitors (hiPSC-CPs)
  • as a component of cryoprotective solution composed of bovine albumin

Other Notes

Formulation contains 10% DMSO.

Legal Information

CryoStor is a registered trademark of BioLife Solutions, Inc.

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This Item
C2999C9249C6295
CryoStor® cell cryopreservation media CS10

Sigma-Aldrich

C2874

CryoStor® cell cryopreservation media

CryoStor® cell cryopreservation media CS5

Sigma-Aldrich

C2999

CryoStor® cell cryopreservation media

CryoSOfree™ DMSO-free Cryopreservation Medium

Sigma-Aldrich

C9249

CryoSOfree™ DMSO-free Cryopreservation Medium

Cell Freezing Medium-DMSO Serum free 1x sterile-filtered, suitable for cell culture

Sigma-Aldrich

C6295

Cell Freezing Medium-DMSO Serum free 1x

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

shipped in

ambient

shipped in

ambient

shipped in

wet ice

shipped in

dry ice

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

Still not finding the right product?

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCZ9889
MKCX4357
MKCX4356
MKCX4355
MKCX4354

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Protocols

CryoStor® Cryopreservation Protocol

Cryopreservation affects post-thaw recovery, viability, and functionality. Stress during freezing and suboptimal media lead to cell death.

Induced Pluripotent Stem Cell Culture Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

Articles

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Cell Culture Fundamentals: Cryopreservation and Storage of Cell Lines

Overview on liquid nitrogen storage and freezing of cell stocks for cell line cryopreservation purposes. Free ECACC handbook download.

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In vivo maturation of human induced pluripotent stem cell-derived cardiomyocytes in neonatal and adult rat hearts
Kadota S, et al.
Stem Cell Reports, 8(2), 278-289 (2017)
Huyen Thi Lam Nguyen et al.
STAR protocols, 1(2) (2020-10-13)
Tumor organoids are promising tools for cancer biology investigations and preclinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor
Susie Barbeau et al.
Stem cell research, 49, 102106-102106 (2020-12-30)
Congenital myasthenic syndromes (CMS) are a class of inherited disorders affecting the neuromuscular junction, a synapse whose activity is essential for movement. CMS with acetylcholinesterase (AChE) deficiency are caused by mutations in COLQ, a collagen that anchors AChE in the
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Global Trade Item Number

SKUGTIN
C2874-100ML04061838259134

Questions

1–4 of 4 Questions  

  1. C2874 is shipped ambient for storage at 2-8oC. How long can the item be kept at room temperature?

    1 answer

    1. This item is stored refrigerated at 2-8°C. However, the product is shipped at ambient temperature. Shipping routes are configured to minimize transit time.

      Refrigerated products that ship at ambient temperature are considered stable up to 2 weeks for transit time.

      Helpful?

  2. Could you please confirm how many units per pack does it contain? Thanks.

    1 answer

    1. This product is supplied in solution as a single 100 mL volume.

      Helpful?

  3. Dear, I am interested in buying the Cryostor CS10, 100 ml (C2874). I am wondering if the product packaging is a bag or a bottle. I can't find the information on the website. In advance, thank you for your answer. Best regards.

    1 answer

    1. This is packaged in a glass bottle with a polyethylene cap.

      Helpful?

  4. Are there any publications available on using product C2874 for freezing human blood neutrophils? What are the viability and functions of frozen neutrophils after thawing?

    1 answer

    1. For information on the viability of neutrophils after freezing and thawing with this product, the following publication can be useful:
      Title: An easy and reliable whole blood freezing method for flow cytometry immuno-phenotyping and functional analyses
      Journal: Cytometry B Clin Cytom. 2021 Nov;100(6):652-665
      DOI: 10.1002/cyto.b.21994
      Published: Epub 2021 Feb 5
      PMID: 33544978

      Helpful?

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