According to Biochim. Biophys. Acta, 271, 400 (1972) the molecular weight of this protein is approximately 10,000 daltons, based on analysis by several techniques (diffusion sedimentation, and gel filtration). A molecular weight of 10,325 daltons has been calculated based on the amino acid composition and amino acid sequence of colipase, which is described in the Journal of Lipid Research, 20, 805-816, (1979).This product is likely to be a mixture of Colipase I and II as described in the following reference: Erlanson, C., et al., Biochim. Biophys. Acta, 310, 437, (1973). This is based on our SDS-PAGE results. The gel pattern shows two major bands, which probably correspond to the two forms of colipases. Minor bands at higher molecular weight may be pro-colipases, or perhaps other unrelated porcine pancreatic proteins.
C3028
Colipase from porcine pancreas
essentially salt-free, lyophilized powder
Synonyme(s) :
Colipase from hog pancreas
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
0.5 mg | Vérifier la disponibilité des articles du panier | 474.00 CHF |
A propos de cet article
biological source
Porcine pancreas
Quality Segment
assay
≥95% (Lowry)
form
essentially salt-free, lyophilized powder
concentration
≥500 μ protein/vial
color
white to faint yellow
solubility
H2O: soluble 1.9-2.1 mg/mL, clear, colorless to faintly yellow
suitability
suitable for molecular biology
UniProt accession no.
application(s)
food and beverages
storage temp.
−20°C
Gene Information
pig ... CLPS(397407)
General description
Colipase is a member of a group of small cysteine-rich proteins characterized by a limited secondary structure. Its structure is relatively flat, measuring 25×30×35 Å, resembling an arrangement of protruding fingers interconnected at one end by a complex network of five disulfide bridges. The colipase structure comprises two small domains with closely related topology.[1]
Application
- in the enzyme solution for lipase assay
- in an enzyme mixture to simulate gastrointestinal digestion
- as one of the apparent critical factors to study its effect on lycopene in vitro accessibility
Biochem/physiol Actions
Features and Benefits
- Overcomes the inhibition of lipase by bile salts.
- Activity can be demonstrated turbidimetrically in an emulsion of triolein and sodium deoxycholate together with porcine pancreatic lipase.
Preparation Note
1 of 1
Cet article | |||
|---|---|---|---|
| description essentially salt-free, lyophilized powder | description powder, ≥400 units/mg protein | description lyophilized, powder, white, ~35 U/mg | description essentially salt-free, lyophilized powder |
| Gene Information pig ... CLPS(397407) | Gene Information pig ... LOC396892(396892) | Gene Information - | Gene Information cow ... CTRB1(618826) |
| biological source Porcine pancreas | biological source Porcine gastric mucosa | biological source Porcine pancreas | biological source bovine pancreas |
| assay ≥95% (Lowry) | assay - | assay - | assay - |
| application(s) food and beverages | application(s) - | application(s) - | application(s) - |
| form essentially salt-free, lyophilized powder | form powder | form powder | form essentially salt-free, lyophilized powder |
| suitability suitable for molecular biology | suitability - | suitability - | suitability - |
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the molecular weight of Product C3028, Colipase from porcine pancreas?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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