While long term stability studies have not been conducted, stock solutions of this material are expected to be stable for at least 6 months, when stored at 2-8°C. Note that product C0663, Acetylcholinesterase from Erythrocytes, a solution prepared in HEPES buffer pH 8.0 w/0.1% TRITON® X-100, is assigned a retest date of approximately 2 years from the date of Quality Release. This item is stored at 2-8°C.
C3389
Acetylcholinesterase from Electrophorus electricus (electric eel)
Type VI-S, lyophilized powder, 200-1,000 units/mg protein
Synonym(s):
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 units | Check Cart for Availability | $121.00 | |
| 2000 units | Please contact Customer Service for Availability | $335.00 | |
| 10000 units | Please contact Customer Service for Availability | $1,140.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.77
EC Number:
232-559-3
MDL number:
Specific activity:
200-1,000 units/mg protein
type
Type VI-S
Quality Level
form
lyophilized powder
specific activity
200-1,000 units/mg protein
mol wt
280 kDa
composition
Protein, ≥45% biuret
solubility
20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
General description
Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)
Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.
Application
Biochem/physiol Actions
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.
Physical form
Lyophilized powder containing Tris buffer salts
Preparation Note
This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.
Analysis Note
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Other Notes
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity 200-1,000 units/mg protein | specific activity ≥900 units/mg protein | specific activity ≥1,000 units/mg protein | specific activity ≥1,000 units/mg protein (BCA) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 280 kDa | mol wt tetramer 440 kDa | mol wt 280 kDa | mol wt 64.6 kDa |
| solubility 20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.) | solubility H2O: soluble 10 mg/mL | solubility Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5) | solubility - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Dan Du et al.
Biosensors & bioelectronics, 25(11), 2503-2508 (2010-05-18)
A simple method to immobilize acetylcholinesterase (AChE) on polypyrrole (PPy) and polyaniline (PANI) copolymer doped with multi-walled carbon nanotubes (MWCNTs) was proposed. The synthesized PAn-PPy-MWCNTs copolymer presented a porous and homogeneous morphology which provided an ideal size to entrap enzyme
Gabriela Villalta et al.
Plants (Basel, Switzerland), 10(6) (2021-07-03)
The essential oil (EO) of Salvia leucantha Cav. was isolated by steam distillation of the aerial parts collected in the South of Ecuador. Its physical properties were evaluated and the chemical composition of the oil was determined by GC-MS and
Jingming Gong et al.
Biosensors & bioelectronics, 24(7), 2285-2288 (2008-12-30)
We developed a simple strategy for designing a highly sensitive electrochemical biosensor for organophosphate pesticides (OPs) based on acetylcholinesterase (AChE) immobilized onto Au nanoparticles-polypyrrole nanowires composite film modifid glassy carbon electrode (labeled as AChE-Au-PPy/GCE). Where, the generated Au nanoparticles (AuNPs)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C3389-2KU | 04061835543335 |
| C3389-10KU | 04061835543328 |
| C3389-500UN | 04061835555918 |
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How can I store this enzyme after dissolving it in Tris Buffer for long term?
1 answer-
Helpful?
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Could you please provide the Uniprot accession number for this product? Also, in general, it would be very helpful if this information were included for any items in your catalog that are proteins. Thanks!
1 answer-
UniProt number O42275 is for Protein Acetylcholinesterase, Organism Electrophorus electricus (Electric eel) (Gymnotus electricus).
Helpful?
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The bottle mentions the concentration as 200-1000 U/mg. What exact concentration should we consider for calculation purposes?
1 answer-
The exact concentration is listed on the lot specific Certificate of Analysis. Please see the sample certificate of analysis: https://www.sigmaaldrich.com/certificates/COFA/C3/C3389/C3389-BULK________SLCJ8803__.pdf
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