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C3766

Cholesterol Esterase from bovine pancreas

lyophilized powder, ≥200 units/g protein

Synonym(s):

Sterol-ester acylhydrolase

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100 units

Available to ship TODAYfromMILWAUKEE

$544.00
500 units

Available to ship TODAYfromMILWAUKEE

$2,890.00
$2,456.50

About This Item

CAS Number:
9026-00-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-808-6
MDL number:
MFCD00071052
EC Number:
3.1.1.13 (BRENDA, IUBMB)
Specific activity:
≥200 units/g protein

$544.00

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form

lyophilized powder

Quality Segment

200

specific activity

≥200 units/g protein

mol wt

84 kDa by SDS-PAGE

composition

protein, ≥20%

storage temp.

−20°C

General description

Cholesterol Esterase from bovine pancreas is a member of α-β hydrolase superfamily. The core of the protein comprises 11 β-strands, surrounded by 15 α-strands. Catalytic triad is in the centre of the molecule. The C-terminus possesses hydrophobic residues.

Application

Cholesterol Esterase from bovine pancreas has been used:
  • in γ-oryzanol-hydrolyzing activity
  • in cholesterol esterase enzyme activity for quantification of cholesterol and cholesterol esters associated in tear film and lenses samples
  • to test in vitro degradation of poly(fumaroyl bioxirane) maleate (PFM) polymer designed for bone tissue engineering

Cholesterol esterase bound to membrane-associated heparin on brush border membranes aids in the transport of cholesterol and free fatty acid across the membrane. This enzyme is widely used in the determination of serum cholesterol in clinical laboratories. The enzyme from Sigma has been used to evaluate the inhibitory and antioxidant functions of the methanol extract of the Camellia sinensis leaves under in vitro conditions. The enzyme has also been used to digest human serum samples to confirm the presence and position of acyl esters of 7α-hydroxycholesterol.
Enzyme responsible for the hydrolysis of many of the fatty acid esters of cholesterol.
Optimum pH range: 6-8
Activators: Bisphenol A diglycidyl ether, cAMP-dependent protein kinase type II, ethanol, methanol, n-butanol, phosphatidylcholine, phosphatidylethanolamine, sodium taurocholic acid
Inhibitors: Bisphenol A methacrylate, diisopropylfluorophosphate, enolase, Hg2+, sodium fluoride, phosphatidic acid, phosphatidylcholine, phosphatidylserine

Biochem/physiol Actions

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. This monomeric glycoprotein may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis. Its molecular mass is found to be 84 kDa.

Physical form

This product is partially purified from bovine pancreas and is supplied as a white to light brown lyophilized powder containing ≥20% protein (biuret) and potassium phosphate.

Preparation Note

Cholesterol esterase is soluble in 0.4 M potassium phosphate, pH 7.0 at 1 mg/mL concentration.

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per minute at pH 7.0 at 37 °C in the presence of taurocholate.
Partially purified

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Cholesterol Esterase from bovine pancreas lyophilized powder, ≥200 units/g protein

Sigma-Aldrich

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Sigma-Aldrich

C1403

Cholesterol Esterase from Pseudomonas sp.

Cholesterol Esterase from Pseudomonas fluorescens lyophilized powder, ≥10,000 units/g protein

Sigma-Aldrich

C9281

Cholesterol Esterase from Pseudomonas fluorescens

Cholesterol Esterase, Microbial Cholesterol Esterase, Microbial, CAS 9026-00-0, is a native enzyme that catalyzes hydrolysis and formation of cholesterol esters, ranging from cholesterol acetate to cholesterol stearate.

Sigma-Aldrich

228180

Cholesterol Esterase, Microbial

description

lyophilized powder, ≥200 units/g protein

description

lyophilized powder, ≥200,000 units/g protein

description

lyophilized powder, ≥10,000 units/g protein

description

Cholesterol Esterase, Microbial, CAS 9026-00-0, is a native enzyme that catalyzes hydrolysis and formation of cholesterol esters, ranging from cholesterol acetate to cholesterol stearate.

specific activity

≥200 units/g protein

specific activity

≥200,000 units/g protein

specific activity

≥10,000 units/g protein

specific activity

≥100 units/mg protein, ≥70 units/mg dry wt.

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

84 kDa by SDS-PAGE

mol wt

~300 kDa

mol wt

~129 kDa

mol wt

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

composition

protein, ≥20%

composition

protein, ≥40% biuret

composition

Protein, ~20%

composition

-

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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