The protein content is determined by the Biuret method. In the product specifications, a protein content >= 70% is guaranteed. As an example, lot number SLCL9662 reports a 90% protein content on the Certificate of Analysis (COA). The impurities are not analyzed. The only other content specification is the concentration of Thymol, <= 0.2% wt.
C40
Catalase from bovine liver
lyophilized powder, ≥10,000 units/mg protein
Synonym(s):
H2O2:H2O2 oxidoreductase
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 mg | Estimated to ship TODAYfromSAINT LOUIS | $85.00 | |
| 500 mg | Estimated to ship TODAYfromSAINT LOUIS | $250.00 | |
| 1 g | Estimated to ship TODAYfromSAINT LOUIS | $430.00 |
About This Item
CAS Number:
eCl@ss:
32160410
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-577-1
MDL number:
Specific activity:
≥10,000 units/mg protein
$85.00
Estimated to ship TODAYDetails
Diagnostics Manufacturers should use alternative grade product: SRE0041
form
lyophilized powder
Quality Level
specific activity
≥10,000 units/mg protein
mol wt
tetramer ~250 kDa
contains
≤0.2 wt. % Thymol
isoelectric point
5.4
solubility
50 mM potassium phosphate buffer, pH 7.0: soluble 2.0 mg/mL
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
SMILES string
O(CC)C(=O)c1ccc(cc1)O
InChI
1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
InChI key
NUVBSKCKDOMJSU-UHFFFAOYSA-N
Gene Information
cow ... CAT(280743)
General description
Research Area: Cell Signaling
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.
Application
Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.
Catalase from bovine liver may be used:
Catalase from bovine liver may be used:
- to prepare H2O2-O2 based biocathode for applications in glucose biofuel cells
- to study the kinetic properties and storage stability of catalase immobilized on to florisil
- in glutathione-mediated superoxide generation in an aqueous solution
Biochem/physiol Actions
Catalase activity is constant over the pH range of 4.0-8.5. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.
Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.
Other Notes
One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.
The protein content is determined by the Biuret method. In the product specifications, a protein content >= 70% is guaranteed. The impurities are not analyzed. The only other content specification is the concentration of Thymol, <= 0.2% wt.
Disclaimer
Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥10,000 units/mg protein | specific activity 2,000-5,000 units/mg protein | specific activity 10,000-40,000 units/mg protein | specific activity 40,000-60,000 units/mg protein (E1%/405) |
| Gene Information cow ... CAT(280743) | Gene Information cow ... CAT(280743) | Gene Information cow ... CAT(280743) | Gene Information cow ... CAT(280743) |
| form lyophilized powder | form powder | form aqueous suspension | form aqueous suspension |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| mol wt tetramer ~250 kDa | mol wt tetramer ~250 kDa | mol wt tetramer ~250 kDa | mol wt tetramer ~250 kDa |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
This procedure may be used for all Catalase products.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 49452-U | 04061837619311 |
| 40147-U | 04061835560493 |
| C40-1G | 04061835515875 |
| C40-500MG | 04061832729138 |
| C40-100MG | 04061835543359 |
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Nelle specifiche leggo che il contenuto proteico di questa catalasi liofilizzata è del 70% Il restante 30% da cosa è composto ?
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