The concentration of catalase C100 varies based on each lot. A sample Certificate of Analysis on the product page shows the mg/mL protein in suspension. Product C9322 is sold by mass, allowing calculation of the required mg/mL to achieve the same concentration. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0. Both products also state their activity in Units/mg protein.
C9322
Catalase from bovine liver
lyophilized powder, 2,000-5,000 units/mg protein
Synonym(s):
H2O2:H2O2 oxidoreductase
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1 G
$65.60
5 G
$139.00
10 G
$239.00
50 G
$843.00
$65.60
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Diagnostics Manufacturers should use alternative grade product: SRE0041
About This Item
CAS Number:
eCl@ss:
32160410
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-577-1
MDL number:
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Product Name
Catalase from bovine liver, lyophilized powder, 2,000-5,000 units/mg protein
SMILES string
O(CC)C(=O)c1ccc(cc1)O
InChI key
NUVBSKCKDOMJSU-UHFFFAOYSA-N
InChI
1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
biological source
bovine liver
form
lyophilized powder
specific activity
2,000-5,000 units/mg protein
mol wt
tetramer ~250 kDa
isoelectric point
5.4
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
cow ... CAT(280743)
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1 of 4
This Item | C40 | C30 | C100 |
|---|---|---|---|
| Gene Information cow ... CAT(280743) | Gene Information cow ... CAT(280743) | Gene Information cow ... CAT(280743) | Gene Information cow ... CAT(280743) |
| biological source bovine liver | biological source - | biological source bovine liver | biological source - |
| specific activity 2,000-5,000 units/mg protein | specific activity ≥10,000 units/mg protein | specific activity 10,000-40,000 units/mg protein | specific activity 40,000-60,000 units/mg protein (E1%/405) |
| form lyophilized powder | form lyophilized powder | form aqueous suspension | form aqueous suspension |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
Application
Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.
Catalase from bovine liver may be used:
Catalase from bovine liver may be used:
- to prepare H2O2-O2 based biocathode for applications in glucose biofuel cells
- to study the kinetic properties and storage stability of catalase immobilized on to florisil
- in glutathione-mediated superoxide generation in an aqueous solution
Biochem/physiol Actions
Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.
This product doesn′t need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.
Disclaimer
Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.
General description
Research Area: Cell Signaling
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.
Other Notes
One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.
Preparation Note
Solutions of catalase should not be frozen. Freezing catalase solutions will lead to a loss of activity by 50-70%. The recommended storage temperature of the product in its powdered form is at -20 °C.
This product is a lyophilized powder with activity of 2,000-5,000 units/mg.
The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.
The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.
related product
Product No.
Description
Pricing
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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Articles
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
Protocols
This procedure may be used for all Catalase products.
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hi, because the catalase C100 is back ordered. we odered the powder of catalase, the catalog number is C9322. we would like to know how to make the powder into aqueous suspension and has the same concentration as catalase C100. thank you!
1 answer-
Helpful?
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How can I reconstitute catalase and how can I store it in the refrigerator?
1 answer-
A 10 mg/mL stock solution prepared in 50 mM potassium phosphate, pH 7.0, and 50% glycerol is stable for several months at 4°C, several weeks at room temperature, and at least 1 week in the freezer at -20°C. However, please note as mentioned in the "Caution" section of the Product Detail Page, freezing catalase solutions will lead to a loss of activity by 50-70%.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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How can Catalase from bovine liver, Product C9322, be reconstituted?
1 answer-
This product is soluble in 50 mM potassium phosphate, pH 7.0 at a concentration of 10 mg/mL.
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What salts are present in Catalase from bovine liver, Product C9322?
1 answer-
Catalase (C9322) contains potassium phosphate buffered salts (and 20% trehalose as a stabilizer).
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