Sepharose 4 Fast Flow is a highly crosslinked 4% agarose matrix which gives excellent physical stability and chromatographic qualities. It was developed to meet high throughput demands of industrial process separations. The rigidity of the resin permits high flow rates, which in turn gives good resolution in a minimum amount of time
C5338
Cyanogen bromide-activated-Sepharose™ 4 Fast Flow
lyophilized powder
Synonym(s):
Cyanogen bromide-activated Agarose
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About This Item
Pricing and availability is not currently available.
form
lyophilized powder
Quality Segment
technique(s)
affinity chromatography: suitable
matrix
Sepharose 4 Fast Flow
matrix active group
cyanate or related structures
matrix spacer
1 atom (when ligands are coupled via isourea derivative or related linkage)
swelling
1 g swells to 4-5 mL gel
particle size
45—165 μm
capacity
6-11 μmol/mL coupling capacity (6-AKS)
storage temp.
2-8°C
General description
C5338-10G′s update product number is GE17-0981-01
Cyanogen bromide-activated Sepharose™ 4 Fast Flow is a resin composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. Proteins and other molecules containing primary amino groups are coupled directly to the preactivated gel. It allows for multi-point attachment of protein ligands which minimizes ligand leakage. The resin is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
Application
Cyanogen bromide-activated-Sepharose™ is used in affinity chromatography and protein chromatography. Cyanogen bromide-activated-Sepharose™ has been used to discover insecticidal agents, to raise polyclonal antibodies against the yeast invertase glycosyls, and to study immunological processes of xenograft rejection.
Physical form
Lyophilized powder with sugar stabilizers
Legal Information
Sepharose is a trademark of Cytiva
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This Item | |||
|---|---|---|---|
| description lyophilized powder | description lyophilized powder | description - | description lyophilized powder |
| matrix active group cyanate or related structures | matrix active group cyanate or related structures | matrix active group - | matrix active group cyanate or related structures |
| particle size 45—165 μm | particle size 45—165 μm | particle size 45-165 μm | particle size - |
| matrix Sepharose 4 Fast Flow | matrix Sepharose 4B | matrix agarose, 4% crosslinked | matrix cross-linked 4% beaded agarose |
| technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable, LPLC: suitable | technique(s) - | technique(s) affinity chromatography: suitable |
| capacity 6-11 μmol/mL coupling capacity (6-AKS) | capacity - | capacity - | capacity ≥10 mg/mL binding capacity (BSA)(via amino groups) |
| form lyophilized powder | form lyophilized powder | form suspension | form lyophilized powder |
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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What does Sepharose 4 Fast Flow mean in the description of Product C5338?
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How do I store Product C5338, Cyanogen bromide?
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This product as it is supplied, is stored at room temperature.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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