The stability of this material following multiple freeze/thaw cycles indicates that a fine precipitate may be evident. However, the performance of the material was is effected. While it is recommended to use this product within 5 days of the initial thaw, it would be up to the end user to continue use following refreezing. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/241/461/c6295pis.pdf
C6295
Cell Freezing Medium-DMSO Serum free 1x
sterile-filtered, suitable for cell culture
Synonym(s):
cryopreservation medium
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| Size/SKU | Availability | Price |
|---|---|---|
50 mL | Check Cart for Availability | $127.00 |
About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
$127.00
Check Cart for Availability
sterility
sterile-filtered
Quality Segment
form
liquid
technique(s)
cell culture | mammalian: suitable, cryopreservation: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
General description
Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.
Application
Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells.
Other Notes
Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.
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This Item | |||
|---|---|---|---|
| description sterile-filtered, suitable for cell culture | description - | description sterile-filtered, suitable for cell culture | description sterile-filtered, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility sterile-filtered |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| impurities endotoxin, tested | impurities - | impurities endotoxin, tested | impurities endotoxin, tested |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Is it possible to thaw this product, prepare aliquots and store them at -20 degrees for further use? Thank you.
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