Key Documents

View All Documentation

Monoclonal Anti-β-Catenin antibody produced in mouse

Name: Monoclonal Anti-β-Catenin antibody produced in mouse
Brand: SIGMA
Price: 705 CAD

clone 15B8, ascites fluid

Synonym(s):

Monoclonal Anti-β-Catenin

Select a Size

Change View

Size/SKU	Availability	Price
100 μL	Please contact Customer Service for Availability	$705.00
0.2 mL	Please contact Customer Service for Availability	$897.00 $762.45

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00282178

Conjugate:

unconjugated

Clone:

15B8, monoclonal

Application:

ARR, ICC, IF, IHC (f), WB

Citations:

100

$705.00

Please contact Customer Service for Availability

A recombinant, preservative-free antibody is available for your target. Try ZRB05665

Request a Bulk Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

15B8, monoclonal

mol wt

antigen 94 kDa

species reactivity

chicken, bovine, human, canine

technique(s)

immunocytochemistry: suitable, immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:1000 using cultured MDBK cells, microarray: suitable, western blot: 1:1000 using cultured MDBK cells extract

isotype

IgG1

UniProt accession no.

P35222

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CTNNB1(1499)

Description

General description

Monoclonal Anti-β-Catenin (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. β-catenin belongs to the armadillo family and comprises the N-terminal region with 12 imperfect repeats and a conserved C-terminal region. The locus 3p22.1 in the human chromosome harbors β-catenin gene. β-catenin shares 70% sequence identity to a protein encoded by Drosophila armadillo, a segment polarity gene. Both armadillo and β-catenin share considerable homology with plakoglobin, which has been proposed to be γ-catenin.

Immunogen

recombinant chicken β-catenin.

Application

Monoclonal Anti-β-Catenin antibody produced in mouse has been used:

immunoblotting[1]
immunohistochemistry[2]
immunolabeling
immunofluorescence[3]
immunocytochemical staining

Biochem/physiol Actions

β-catenin is a prime component of the wingless (Wnt) pathway and plays a key role in gene expression during development. The homology between β-catenin and armadillo raised the possibility that β-catenin has a developmental signaling role in vertebrates. β-catenin mediates the interaction of the E-cadherin-catenin complex with the epidermal growth factor (EGF) receptor. β-catenin and plakoglobin are substrates for tyrosine phosphorylation following EGF stimulation of cells. β-catenin also associates with the tumor suppressor protein adenomatous polyposis coli (APC). Monoclonal antibody reacting specifically with β-catenin is an essential tool in defining the interactions and distributions of β-catenin and its relationships with other catenins and cadherins in various cells and tissues.

Recognizes specifically mammalian and avian β-catenin. Does not cross-react with plakoglobin (γ-catenin).

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Preparation Note

For extended storage freeze in working aliquots. For continuous use, store at 2-8 °C for up to one month. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	SAB4701035	SAB4200720	C7738
Sigma-Aldrich C7207 Monoclonal Anti-β-Catenin antibody produced in mouse	Sigma-Aldrich SAB4701035 Monoclonal Anti-betaCatenin antibody produced in mouse	Sigma-Aldrich SAB4200720 Anti-β-Catenin antibody, Mouse monoclonal	Sigma-Aldrich C7738 Anti-β-Catenin−Cy3 antibody, Mouse monoclonal
species reactivity chicken, bovine, human, canine	species reactivity -	species reactivity chicken, mouse, human, rat, monkey, hamster, bovine, canine	species reactivity chicken, bovine, human, canine
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate CY3 conjugate
Gene Information human ... CTNNB1(1499)	Gene Information human ... CTNNB1(1499)	Gene Information human ... CTNNB1(1499) mouse ... Ctnnb1(12387) rat ... Ctnnb1(84353)	Gene Information human ... CTNNB1(1499)
biological source mouse	biological source mouse	biological source mouse	biological source mouse
antibody form ascites fluid	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin
clone 15B8, monoclonal	clone EM-22, monoclonal	clone 15B8, monoclonal	clone 15B8, monoclonal

Still not finding the right product?

Explore all of our products under Monoclonal Anti-β-Catenin antibody produced in mouse

— or —

Try our Product Selector Tool to narrow your options

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.