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C7774

Human Collagen Type I

from human placenta, powder, suitable for cell culture

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5 mg
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$252.00
25 mg
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$993.00

About This Item

CAS Number:
9007-34-5
UNSPSC Code:
12352202
NACRES:
NA.61
EC Number:
232-697-4
MDL number:
MFCD00130825
Form:
powder
Biological source:
human placenta

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Product Name

Collagen from human placenta, Bornstein and Traub Type I (Sigma Type VIII), powder

biological source

human placenta

Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

HIV, hepatitis B and hepatitis C, none detected

UniProt accession no.

storage temp.

2-8°C

Gene Information

Application

This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Biochem/physiol Actions

Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.

Preparation Note

Prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.

Other Notes

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

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C5533H4417C8374
Collagen from human placenta Bornstein and Traub Type I (Sigma Type VIII), powder

Sigma-Aldrich

C7774

Collagen from human placenta

Collagen from human placenta Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture

Sigma-Aldrich

C5533

Collagen from human placenta

Collagen from human placenta Bornstein and Traub Type IV, solution, suitable for cell culture, High Performance

Sigma-Aldrich

H4417

Collagen from human placenta

Collagen from human placenta Bornstein and Traub Type IV, powder

Sigma-Aldrich

C8374

Collagen from human placenta

description

Bornstein and Traub Type I (Sigma Type VIII), powder

description

Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture

description

Bornstein and Traub Type IV, solution, suitable for cell culture, High Performance

description

Bornstein and Traub Type IV, powder

biological source

human placenta

biological source

human placenta

biological source

human placenta

biological source

human placenta

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

form

powder

form

powder

form

solution

form

powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

UniProt accession no.

P02668, P53420, P02666, Q01955, Q14031

UniProt accession no.

P02462, P29400, P08572, P53420, Q01955, Q14031

UniProt accession no.

P53420, Q14031, Q01955, P29400, P02462, P08572

UniProt accession no.

P08572, P53420, P02462, Q14031, Q01955, P29400

Gene Information

bovine ... CSN2(281099), CSN3(281728)
human ... COL4A3(1285), COL4A4(1286), COL4A6(1288)

Gene Information

human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

Gene Information

human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

Gene Information

human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

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Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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Questions

1–3 of 3 Questions  

  1. El producto es soluble en PBS 1X con 0.5 M de ácido acético o es preferible el uso de agua?

    1 answer

    1. This product is an acid-soluble collagen. Prepare the stock solution in 0.5 M acetic acid. Alternatively, dissolve this product in water and adjust the pH to 3.0 with acetic acid at a concentration of up to 5 mg/mL. The stock solution can then be further diluted with the required buffer, such as phosphate-buffered saline. Direct dissolution in phosphate-buffered saline is not recommended.

      Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/348/610/c7774pis.pdf

      Helpful?

  2. Is Human Collagen Type I, (Cat # C7774) isolated enzymatically or by acid extraction? Atelo or Telo?

    1 answer

    1. As mentioned in the Preparation Note on the product webpage, the collagen is "prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.
      The presence of Atelo or Telo collagen is not determined.

      Helpful?

  3. 해당 제품의 순도가 궁금합니다.

    1 answer

    1. Unfortunately, the purity of this product is not determined. An SDS-PAGE is performed and the electrophoretic profile is confirmed to be consistent with past lots. For additional information please see the link below to the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/348/610/c7774pis.pdf

      Helpful?

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