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MilliporeSigma

C5533

Sigma-Aldrich

Human Collagen Type IV

from human placenta, powder, suitable for cell culture

Collagen from human placenta

Synonym(s):

Collagen Type 4

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$199.00

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5 MG
$199.00

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.75

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$199.00


In StockDetails


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Product Name

Collagen from human placenta, Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture

biological source

human placenta

Quality Level

product line

BioReagent

form

powder

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

impurities

HIV, hepatitis B and hepatitis C, none detected

solubility

acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)

binding specificity

Peptide Source: Collagen Type IV

storage temp.

−20°C

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1 of 4

This Item
H4417C7521C8374
biological source

human placenta

biological source

human placenta

biological source

human placenta

biological source

human placenta

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | stem cell: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

solution

form

powder

form

powder

packaging

pkg of 5 mg

packaging

-

packaging

-

packaging

-

binding specificity

Peptide Source: Collagen Type IV

binding specificity

-

binding specificity

-

binding specificity

-

General description

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains. Type IV collagens are localized to the basement membrane and they are network-forming proteins. It is encoded by six different genes: collagen type IV α1 (COL4A1), COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6. Both COL4A1 and COL4A2 genes are mapped to human chromosome 13q34. The proteins encoded by the two genes share 45% homology. They contain: a non-collagenous (NC1) domain, triple-helical collagenous domain and Gly-Xaa-Yaa repeats in common.[1] Both COL4A3 and COL4A4 genes are located on human chromosome 2q36.3.[2] In tissues, type IV collagens are present as procollagens.[3]

Application

Collagen from human placenta has been used:
  • To solubilize extracellular matrix for surface functionalization.[4]
  • To induce endothelial cell [5] and rat pluripotent cell differentiation.[6]
  • As an adherent in cell migration/invasion assay.[7]
  • To assess the bioelectric effects of quinine on airway epithelial cells.
  • To study the selective toxicity of engineered lentvirus lytic peptides.
  • In particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus.
  • In a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.

Biochem/physiol Actions

Type IV collagen maintains cell division and growth.[3] Abnormal collagen secretion affects basement membrane formation.[8] The collagen type IV, α1 (COL4A1) gene is overexpressed in gastric cancer.[9] Mutation in COL4A1 contributes to intracerebral hemorrhage in adults and children.[10] Cerebrovascular disease is resulted due to COL4A1 and COL4A2 mutations.[1] Type IV collagen has been found to play a key role in angiogenesis, neurological diseases and metastasis.
During development, collagen IV is ubiquitously distributed in the basement membranes (BMs). During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining the BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.
Tissue injury in the autoimmune disease, Goodpasture syndrome, is due to the pathogenic autoantibodies targeting the collagen IV α3 chain. Mutations in the COL4A5 gene are associated with Alport syndrome.

Caution

This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.

Preparation Note

This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It was prepared by a modification of the pepsin extraction method of Niyibizi, C. et. al

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Questions

1–2 of 2 Questions  
  1. Hi, I have several questions about collagen coating. 1.when we dissolve the collagenIV powder in 0.5M acetic acid, can it be stored at -30 degree? 2. When we dilute the collagen stock with 0.25% acetic acid, how long can i store it at 4 degree?

    1 answer
    1. Solutions of 1 -2 mg/mL collagen in 0.5M acetic acid should be stable for at least 1 year at -20°C.
      Collagen solutions stored at 2-8°C should remain stable only for a few days up to 1 week.

      Helpful?

  2. Goodmorning, I noticed that in the upper instructions it is stated to use acetic acid but below it seems to be also possible to use water/PBS. What holds true? And is the powder itself stable?

    1 answer
    1. As mentioned on the product page under 'Preparation Note'' in the DESCRIPTION section, this lyophilized powder can be reconstituted in sterile 0.5 M acetic acid, water, or PBS to a concentration of 1 mg/mL. The product is tested for solubility in acetic acid at 1 mg/mL. The powder is not assigned an expiration date. It is assigned a retest schedule of every 3 years and is quite stable if stored properly.

      Please see the link below to review the product data sheet for more information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/259/550/c5533pis.pdf

      Helpful?

Reviews

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  1. Charlottesville, VA
    • Reviews 2
    • Votes 3
    5 out of 5 stars.

    Functional protein for primary cell culture

    I ordered this product as recommended by a protocol for use in a plate coating buffer for primary cell culture. It is a functional protein and supports epithelial cell growth.

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