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Monoclonal Anti-Collagen, Type X antibody produced in mouse

Name: Monoclonal Anti-Collagen, Type X antibody produced in mouse
Brand: SIGMA

clone COL-10, ascites fluid

Synonym(s):

Anti-Col10, Anti-Col10a-1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02686071

Conjugate:

unconjugated

Clone:

COL-10, monoclonal

Application:

DB, ICC, WB

Citations:

Pricing and availability is not currently available.

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

COL-10, monoclonal

mol wt

antigen 60 kDa (in denatured-reduced preparations)

contains

15 mM sodium azide

species reactivity

deer, human, pig

technique(s)

dot blot: suitable, immunocytochemistry: 1:1,000 using HT 1080 human fibrosarcoma cells, western blot: suitable

isotype

IgM

UniProt accession no.

Q03692

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... COL10A1(1300)

Description

General description

Collagens are extracellular glycoproteins made up of three polypeptides that intermingle to form a triple helix. Type X collagen is a homotrimer of 59 kD α1(X) chains found in fetal hypertrophic cartilage in the growth zones of long bones, vertebrae and ribs, whereas in adults it is also present in thyroid cartilage. Monoclonal anti-collagen, type X antibody can be used in histological and immunohistochemical evaluation of cell cultures. It is also useful in study of specific differential tissue expression.

Monoclonal Anti-Collagen, Type X (mouse IgM isotype) is derived from the COL-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with porcine collagen type X. Collagen type X shares a similar domain structure with type VIII collagen: a central triple-helical (COL1) domain of 50 kDa is flanked by N-terminal (NC2) and C-terminal (NC1) non-triple-helical domains.

Immunogen

purified pig collagen type X.

Application

Monoclonal Anti-Collagen, Type X antibody produced in mouse has been used in

enzyme linked immunosorbent assay (ELISA)
dot-blot
immunoblotting and
immunohistochemistry

Biochem/physiol Actions

Type X collagen is a product of hypertrophic chondrocytes. Type X collagen is non-fibrillar, but forms fine pericellular filaments in association with cartilage collagen. It interacts with matrix proteins, such as connexin V, chondrocalcein, collagen II and proteoglycans, as well as with Ca²⁺. It acts as a scaffold to avoid local collapse during endochondral ossification and also has a role in cartilage mineralization. Mutations in the NC1-encoding domain of the human α1(X) collagen gene, are associated with Schmid metaphysical chondroplasia.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C7805	C2456	SAB4200749
Sigma-Aldrich C7974 Monoclonal Anti-Collagen, Type X antibody produced in mouse	Sigma-Aldrich C7805 Monoclonal Anti-Collagen, Type III antibody produced in mouse	Sigma-Aldrich C2456 Monoclonal Anti-Collagen, Type I antibody produced in mouse	Sigma-Aldrich SAB4200749 Anti-Collagen Type III antibody, Mouse monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone COL-10, monoclonal	clone FH-7A, monoclonal	clone COL-1, monoclonal	clone FH-7A, monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate -
species reactivity deer, human, pig	species reactivity human, rat	species reactivity bovine, human, pig, rat, rabbit, deer	species reactivity rat, human
Gene Information human ... COL10A1(1300)	Gene Information human ... COL3A1(1281) rat ... Col3a1(84032)	Gene Information rat ... Col1a1(29393)	Gene Information human ... COL3A1(1281)
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form purified from hybridoma cell culture

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c