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C8058

Chondroitinase B from Flavobacterium heparinum

lyophilized powder (with BSA as stabilizer)

Synonym(s):

Chondroitin sulfate B lyase

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50 units
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$786.00

About This Item

CAS Number:
52227-83-5
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00166444
Biological source:
bacterial (Flavobacterium heparinum)

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biological source

bacterial (Flavobacterium heparinum)

Quality Segment

conjugate

(Glucosaminoglycan)

form

lyophilized powder (with BSA as stabilizer)

shipped in

dry ice

storage temp.

−20°C

Application

The enzyme from Sigma has been used for the analysis of the frequency of iduronic acid in dermatan sulfate dodecasaccharide. It has also been used to digest dermatan sufate (DS) in melanoma cells. This digestion resulted in decreased proliferation and invasiveness of tumor cells, thereby suggesting a role for DS in metastasis.
Chondroitinase B from Flavobacterium heparinum has been used in a study to assess the structural characterization and antithrombin activity of dermatan sulfate. Chondroitinase B from Flavobacterium heparinum has also been used in a study to investigate the chondroitin lyase action pattern via liquid chromatography–mass spectrometry.

Biochem/physiol Actions

Chondroitinase B degrades only chondroitin sulphate B producing oligo- and tetra-saccharides, and an unsaturated 4-sulphated disaccharide. It has an optimum temperature of 20 °C and an optimum pH of 8.0. The enzyme activity is inhibited by 50% using 0.1 M NaCI. Co2+, Fe3+ and Ba2+ also inhibit its activity.

Other Notes

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25°C using chondroitin sulfate B as substrate.

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This Item
E2039H3917C3667
Chondroitinase B from Flavobacterium heparinum lyophilized powder (with BSA as stabilizer)

Sigma-Aldrich

C8058

Chondroitinase B from Flavobacterium heparinum

Chondroitinase AC from Flavobacterium heparinum recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis

Sigma-Aldrich

E2039

Chondroitinase AC from Flavobacterium heparinum

Heparinase I and III Blend from Flavobacterium heparinum lyophilized powder, stabilized with ∼ 25% (w/w) bovine serum albumin, ≥200 unit/mg protein (enzyme + BSA)

Sigma-Aldrich

H3917

Heparinase I and III Blend from Flavobacterium heparinum

Chondroitinase ABC from Proteus vulgaris BSA free, lyophilized powder, specific activity 50-250 units/mg protein

Sigma-Aldrich

C3667

Chondroitinase ABC from Proteus vulgaris

description

lyophilized powder (with BSA as stabilizer)

description

recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis

description

lyophilized powder, stabilized with ∼ 25% (w/w) bovine serum albumin, ≥200 unit/mg protein (enzyme + BSA)

description

BSA free, lyophilized powder, specific activity 50-250 units/mg protein

biological source

bacterial (Flavobacterium heparinum)

biological source

-

biological source

bacterial (Flavobacterium heparinum)

biological source

bacterial (Proteus vulgaris)

form

lyophilized powder (with BSA as stabilizer)

form

lyophilized solid

form

lyophilized powder

form

lyophilized powder

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

conjugate

(Glucosaminoglycan)

conjugate

(Glucosaminoglycan)

conjugate

conjugate (Glucosaminoglycan)

conjugate

(Glucosaminoglycan)

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Codes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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