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C8374

Human Collagen Type IV

from human placenta, powder

Synonym(s):

Collagen from human placenta, Collagen Type 4

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5 mg
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$519.00
$441.15

About This Item

CAS Number:
9007-34-5
UNSPSC Code:
12352202
NACRES:
NA.61
EC Number:
232-697-4
MDL number:
MFCD00130825
Form:
powder
Assay:
>85% (SDS-PAGE)
Biological source:
human placenta

$441.15

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Product Name

Collagen from human placenta, Bornstein and Traub Type IV, powder

biological source

human placenta

Quality Level

200

assay

>85% (SDS-PAGE)

form

powder

technique(s)

cell culture | stem cell: suitable

impurities

HIV, hepatitis B and hepatitis C, none detected

solubility

0.5 M acetic acid: 1 mg/mL, clear to very slightly hazy

UniProt accession no.

P02462, P08572, P29400, P53420, Q01955, Q14031

storage temp.

−20°C

Gene Information

human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

Application

Type IV collagen has been found to play a key role in angiogenesis, neurological diseases and metastasis. Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.

Biochem/physiol Actions

During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.

Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain . Mutations in COL4A5 are associated with Alport syndrome.

Preparation Note

This powder can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C.

Analysis Note

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Other Notes

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Disclaimer

This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.

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Collagen from human placenta Bornstein and Traub Type IV, powder

Sigma-Aldrich

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Collagen from human placenta Bornstein and Traub Type III (Sigma Type X), powder

Sigma-Aldrich

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Collagen from human placenta

Sigma-Aldrich

Sigma-Aldrich

234149

Collagen, Type I, Human Placenta

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

assay

>85% (SDS-PAGE)

assay

-

assay

≥90% (GE)

assay

≥90% (SDS-PAGE)

biological source

human placenta

biological source

human placenta

biological source

human placenta

biological source

-

form

powder

form

solution

form

powder

form

solution

solubility

0.5 M acetic acid: 1 mg/mL, clear to very slightly hazy

solubility

-

solubility

aqueous acid: soluble

solubility

-

Gene Information

human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

Gene Information

human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

Gene Information

human ... COL3A1(1300)

Gene Information

-

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000483073
0000483073
0000481306
0000481306
SLCN7690

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Jian Liu et al.
Stem cell research & therapy, 11(1), 348-348 (2020-08-14)
The biological role of miR-203 and the underlying mechanisms on the proliferation of epidermal stem cells (ESCs) have not yet been reported during the progression of chronic wound healing in diabetes mellitus. Our previous studies have observed that the expression
R Kalluri et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(13), 6201-6205 (1994-06-21)
Human Goodpasture syndrome is a lethal form of autoimmune disease that is characterized by pulmonary hemorrhage and glomerulonephritis. The tissue injury is mediated by autoantibodies that bind to glomerular and alveolar basement membrane. The target autoantigen is alpha 3(IV) collagen
Type IV collagen: structure, gene organization, and role in human diseases. Molecular basis of Goodpasture and Alport syndromes and diffuse leiomyomatosis.
B G Hudson et al.
The Journal of biological chemistry, 268(35), 26033-26036 (1993-12-15)
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Global Trade Item Number

SKUGTIN
C8374-5MG04061833522189

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