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C8482

Protein Kinase A catalytic subunit human

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Synonym(s):

Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human

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10 μg
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$1,010.00

About This Item

UNSPSC Code:
41106305
NACRES:
NA.32
MDL number:
MFCD03458215
Specific activity:
>1000 units/mg protein
Assay:
>90% (SDS-PAGE)
Recombinant:
expressed in E. coli

$1,010.00

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recombinant

expressed in E. coli

Quality Level

200

assay

>90% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

>1000 units/mg protein

mol wt

43.5 kDa

UniProt accession no.

P17612

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKACA(5566)

Biochem/physiol Actions

Phosphorylates a spectrum of substrates, and regulates many cellular processes.
Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

Physical form

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

Analysis Note

One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.

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Protein Kinase A catalytic subunit human >90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Sigma-Aldrich

C8482

Protein Kinase A catalytic subunit human

Protein Kinase A Catalytic Subunit β, Active human recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

Sigma-Aldrich

P6998

Protein Kinase A Catalytic Subunit β, Active human

Protein kinase CβII isozyme human >85% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

Sigma-Aldrich

P3287

Protein kinase CβII isozyme human

Protein Kinase Cδ isozyme human >95% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

Sigma-Aldrich

P8538

Protein Kinase Cδ isozyme human

specific activity

>1000 units/mg protein

specific activity

≥10 nmol/min·mg

specific activity

≥500 unit/mg solid

specific activity

-

assay

>90% (SDS-PAGE)

assay

≥80% (SDS-PAGE)

assay

>85% (SDS-PAGE)

assay

>95% (SDS-PAGE)

Gene Information

human ... PRKACA(5566)

Gene Information

human ... PRKACB(5567)

Gene Information

human ... PRKCB(5579)

Gene Information

human ... PRKCD(5580)

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected insect cells

UniProt accession no.

P17612

UniProt accession no.

P22694

UniProt accession no.

P05771

UniProt accession no.

Q05655

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ3062
SLBH3769
SLBG4521
SLBD2602
021M0424

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APOBEC3B cytidine deaminase (A3B) catalyzes cytosine into uracil in single-strand DNA and induces C-to-T mutations in genomic DNA of various types of tumors. Accumulation of APOBEC signature mutations is correlated with a worse prognosis for patients with breast cancer or

Global Trade Item Number

SKUGTIN
C8482-10UG04061833502778

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