Key Documents

Anti-Caspase 3, Active antibody produced in rabbit

Name: Anti-Caspase 3, Active antibody produced in rabbit
Brand: SIGMA
Price: 201 USD
Availability: InStock

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Activated Caspase 3 Antibody, Activated Caspase 3 Antibody - Anti-Caspase 3, Active antibody produced in rabbit, Anti-Apopain, Anti-CPP32, Anti-Yama

Select a Size

Change View

Size/SKU	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$201.00
100 μL	Available to ship TODAYfromMILWAUKEE	$580.00
200 μL	Available to ship TODAYfromMILWAUKEE	$727.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01321906

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IF, WB

Citations:

129

$201.00

Available to ship TODAYDetails

Request a Custom Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, bovine, human, pig, canine, mouse

packaging

antibody small pack of 25 μL

technique(s)

indirect immunofluorescence: 1:1,000 using human epitheloid carcinoma HeLa cell line, treated with staurosporine, microarray: suitable, western blot: 1:500 using recombinant human caspase 3, active (Sigma Product No. C1224)

UniProt accession no.

P42574

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CASP3(836)
mouse ... Casp3(12367)
rat ... Casp3(25402)

Description

General description

Caspase-3 (CASP3) belongs to the class of cysteine proteases. CASP3 is an executioner caspase, which is cleaved and activated with the help of caspase-8 and caspase-9 initiator caspases. CASP3 is mapped to human chromosome 4q35.1.

Caspases are a family of intracellular proteases that mediate cell death and the principal effectors of apoptosis. There are 14 different caspases have identified which can be grouped into three different subfamilies based on their substrate specificities. Caspase 3 is also known as CPP32, Yama, apopain. Among all the types, caspase 3, a cytosolic protein, found in cells as an inactive 32 kDa proenzyme.

Immunogen

Synthetic peptide corresponding to the cleavage site of human caspase 3 conjugated to KLH.

Application

Anti-Caspase 3, Active antibody has been used:

for western blotting of cytochrome c for caspase activation
as primary antibody in immunofluorescence staining of embryos and postnatal mice cryosections
in Western blot analysis of activated caspase 3
as a primary antibody in immunodetection of rat brain sections

Biochem/physiol Actions

Caspase 3 is one of the key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD). Caspase 3 brings about cell death by activating CAD (caspase activated DNAse I), an endonuclease that causes degradation of chromosomal DNA.

Physical form

Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20 °C. For continuous use, the product may be storage, the product may be stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	C7729	C5737	SAB5700153
$Anti-Caspase 3, Active antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C8487 Anti-Caspase 3, Active antibody produced in rabbit	Sigma-Aldrich C7729 Anti-Caspase 9 antibody produced in rabbit	Sigma-Aldrich C5737 Monoclonal Anti-Caspase 3 antibody produced in mouse	Sigma-Aldrich SAB5700153 Anti-Caspase antibody produced in rabbit
description IgG fraction of antiserum, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description clone 84803.111, purified immunoglobulin, lyophilized powder	description -
antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
Gene Information human ... CASP3(836) mouse ... Casp3(12367) rat ... Casp3(25402)	Gene Information human ... CASP9(842) rat ... Casp9(58918)	Gene Information human ... CASP3(836)	Gene Information human ... CASP3(836)
form buffered aqueous solution	form buffered aqueous solution	form lyophilized powder	form buffered aqueous solution

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

12 - Non Combustible Liquids

wgk

nwg

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.