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Concanavalin A from Canavalia ensiformis (Jack bean)

Name: Concanavalin A from <I>Canavalia ensiformis</I> (Jack bean)
Brand: SIGMA
Price: 694 CAD

Sepharose^™ conjugate, buffered aqueous suspension

Synonym(s):

ConA

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Pack Size	SKU	Availability	Price
25 mL		Please contact Customer Service for Availability	$694.00
100 mL		Please contact Customer Service for Availability	$2,260.00

About This Item

NACRES:

NA.32

UNSPSC Code:

12161501

Form:

buffered aqueous suspension

Biological source:

Canavalia ensiformis

$694.00

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Properties

Quality Segment

200

biological source

Canavalia ensiformis

conjugate

Sepharose^™ conjugate

form

buffered aqueous suspension

contains

20% ethanol as preservative

extent of labeling

10-16 mg per mL

matrix

Sepharose 4B

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥25 mg/mL binding capacity (thyroglobulin)

storage temp.

2-8°C

Description

Application

Used for the purification of glycoproteins containing mannose.

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Physical form

Suspension in 0.1 M acetate buffer, pH 6.0, containing 1 M NaCl, and 1 mM each of CaCl₂ , MgCl₂, and MnCl₂

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Legal Information

Sepharose is a trademark of Cytiva

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This Item	C7555	C7642	L7647
Sigma-Aldrich C9017 Concanavalin A from Canavalia ensiformis (Jack bean)	Sigma-Aldrich C7555 Concanavalin A from Canavalia ensiformis (Jack bean)	Sigma-Aldrich C7642 Concanavalin A from Canavalia ensiformis (Jack bean)	Sigma-Aldrich L7647 Concanavalin A from Canavalia ensiformis (Jack bean)
biological source Canavalia ensiformis	biological source Canavalia ensiformis	biological source Canavalia ensiformis	biological source Canavalia ensiformis
form buffered aqueous suspension	form saline suspension	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
matrix Sepharose 4B	matrix cross-linked 4% beaded agarose	matrix -	matrix -
conjugate Sepharose^™ conjugate	conjugate agarose conjugate	conjugate FITC conjugate	conjugate -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200