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Concanavalin A from Canavalia ensiformis (Jack bean)

Name: Concanavalin A from <I>Canavalia ensiformis</I> (Jack bean)
Brand: SIGMA
Price: 580 USD
Availability: InStock

Sepharose^™ conjugate, buffered aqueous suspension

Synonym(s):

ConA

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Size/SKU	Availability	Price
25 mL	Check Cart for Availability	$580.00
100 mL	Check Cart for Availability	$1,960.00

About This Item

NACRES:

NA.32

UNSPSC Code:

12161501

Form:

buffered aqueous suspension

Biological source:

Canavalia ensiformis

$580.00

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Properties

Quality Segment

200

biological source

Canavalia ensiformis

conjugate

Sepharose^™ conjugate

form

buffered aqueous suspension

contains

20% ethanol as preservative

extent of labeling

10-16 mg per mL

matrix

Sepharose 4B

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥25 mg/mL binding capacity (thyroglobulin)

storage temp.

2-8°C

Description

Application

Used for the purification of glycoproteins containing mannose.

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Physical form

Suspension in 0.1 M acetate buffer, pH 6.0, containing 1 M NaCl, and 1 mM each of CaCl₂ , MgCl₂, and MnCl₂

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Legal Information

Sepharose is a trademark of Cytiva

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This Item	L7647	C5275	C0412
Sigma-Aldrich C9017 Concanavalin A from Canavalia ensiformis (Jack bean)	Sigma-Aldrich L7647 Concanavalin A from Canavalia ensiformis (Jack bean)	Sigma-Aldrich C5275 Concanavalin A from Canavalia ensiformis (Jack bean)	Sigma-Aldrich C0412 Concanavalin A from Canavalia ensiformis (Jack bean)
description Sepharose^™ conjugate, buffered aqueous suspension	description Type VI, lyophilized powder	description Type IV-S, lyophilized powder, aseptically processed, BioReagent, suitable for cell culture	description Type IV-S, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture
biological source Canavalia ensiformis	biological source Canavalia ensiformis	biological source -	biological source -
form buffered aqueous suspension	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
matrix Sepharose 4B	matrix -	matrix -	matrix -
conjugate Sepharose^™ conjugate	conjugate -	conjugate -	conjugate -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200