As mentioned in the 'DESCRIPTION'' section, under 'Preparation Note', a solution of collagenase can be prepared at 1-2 mg/mL in TESCA buffer (containing 50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37°C).
C9407
Collagenase from Clostridium histolyticum
powder, Suitable for the digestion and isolation of physiologically active pancreatic islet cells, suitable for cell culture
Synonym(s):
Clostridiopeptidase A
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| Size/SKU | Availability | Price |
|---|---|---|
25 mg | Check Cart for Availability | $91.10 |
100 mg | Check Cart for Availability | $201.00 |
500 mg | Estimated to ship onNovember 18, 2026fromMILWAUKEE | $880.00 |
1 g | Check Cart for Availability | $1,350.00 |
5 g | Check Cart for Availability | $4,700.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.75
EC Number:
232-582-9
MDL number:
EC Number:
Specific activity:
≥800 units/mg solid
Biological source:
Clostridium histolyticum
biological source
Clostridium histolyticum
Quality Segment
form
powder
specific activity
≥800 units/mg solid
mol wt
68-130 kDa
packaging
pkg of 1 μmol
technique(s)
cell culture | mammalian: suitable
pH
7.4
storage temp.
−20°C
General description
Collagenase enzyme from Clostridium histolyticum has collagen binding domain (CBD) and polycystic kidney disease-like (PKD-like) domains. It also has Ca2+ binding site for calcium binding, which is crucial for its functionality. Collagenase is used in the management of connective tissue disorder, Dupuytren′s contracture.
Application
This product is suitable for the disaggregation of human tumor, mouse kidney, human adult and fetal brain, lung and many other epithelia tissues. It has also been shown to be effective in liver and kidney perfusion studies, digestion of pancreas, isolation of nonparenchymal rat liver cells and hepatocyte preparation. Collagenase has also been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products. This enzyme has been tested for the release of heptatocytes at a concentration of approximately 1 mg/mL. Concentrations for digestion range from 0.1 to 5 mg/mL.
Collagenase from Clostridium histolyticum has been used in the digestion:
- of pancreatic samples for islets isolation
- of pulmonary artery samples for pulmonary vascular smooth muscle cells (PVSMCs) isolation
- of cartilage samples for chondrocytes isolation
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, and a neutral protease. The synergistic action of these enzymes degrade collagen and other intracellular materialThe action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.
Preparation Note
This proudct is prepared from Type XI (C7657) Collagenase from Clostridium histolyticum. It also contains clostripain, nonspecific neutral protease and tryptic activities. Solutions are typically prepared at 1-2 mg/mL in TESCA buffer (containing 50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37°C.
Other Notes
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Disclaimer
As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.
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This Item | |||
|---|---|---|---|
| description powder, Suitable for the digestion and isolation of physiologically active pancreatic islet cells, suitable for cell culture | description powder, suitable for cell culture, ≥4 FALGPA units/mg solid, high purity, ≥700 CDU/mg solid (CDU = collagen digestion units) | description Sigma Blend Type F, ≥2.0 FALGPA units/mg solid | description high purity, purified by chromatography, Type VII, ≥4 FALGPA units/mg solid, lyophilized powder, ≥700 CDU/mg solid (CDU = collagen digestion units) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - |
| specific activity ≥800 units/mg solid | specific activity ≥700 CDU/mg solid (CDU = collagen digestion units), ≥4 FALGPA units/mg solid | specific activity ≥2.0 FALGPA units/mg solid | specific activity ≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units) |
| biological source Clostridium histolyticum | biological source Clostridium histolyticum | biological source Clostridium histolyticum | biological source - |
| form powder | form powder | form powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| pH 7.4 | pH 7.4 | pH - | pH - |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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