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MRC-5 (PD 30) Cell Line human

Name: MRC-5 (PD 30) Cell Line human
Brand: SIGMA

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 5090501, human lung, Fibroblast-like

Synonym(s):

MRC-5 (PD30) Cells

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About This Item

UNSPSC Code:

41106514

Biological source:

human lung

Growth mode:

Adherent

Karyotype:

2n=46, diploid

Morphology:

Fibroblast-like

Manufacturer/tradename:

ECACC

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Properties

biological source

human lung

description

Human fetal lung

manufacturer/tradename

ECACC

packaging

tube of 5 μg 05090501-DNA-5UG, pkg of vial of cells 05090501-1VL

growth mode

Adherent

karyotype

2n=46, diploid

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Description

Biochem/physiol Actions

Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.

Human foetal lung

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

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This Item	CB_85020203	CB_90110505	CB_88011502
MRC-5 (PD 30) Cell Line human	MRC-7	GS-109-V-20	R9ab
description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 5090501, human lung, Fibroblast-like	description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.	description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.	description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
description Human fetal lung	description -	description -	description -
manufacturer/tradename ECACC	manufacturer/tradename ECACC	manufacturer/tradename ECACC	manufacturer/tradename ECACC
storage temp. −196°C	storage temp. −196°C	storage temp. −196°C	storage temp. −196°C
karyotype 2n=46, diploid	karyotype 2n = 46, diploid	karyotype 2n = 46	karyotype 2n = 44
morphology Fibroblast-like	morphology Fibroblast	morphology Fibroblast-like	morphology Fibroblast-like

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