This resin can be autoclaved for 20 minutes at 120°C in phosphate buffer at pH 7.
CL2B300
Sepharose™ CL-2B Size Exclusion Resin
Cross-linked, 70-230 mesh
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 mL | Check Cart for Availability | $325.00 | |
| 500 mL | Check Cart for Availability | $1,600.00 |
About This Item
Product Name
Sepharose™ CL-2B, Cross-linked
Quality Segment
form
suspension
bead diameter
60-200 μm
pore size
100,000-20,000,000 fractionation range (Dextrans), 70,000-40,000,000 fractionation range (Globular proteins)
storage temp.
2-8°C
General description
CL2B300-1L′s updated product number is GE17-0140-01
Application
Sepharose CL-2B is used in affinity chromatography, gel filtration chromatography, protein chromatography, separation media and resins. Sepharose CL-2B has been used to study ovarian carcinoma and cystic fibrosis.
Sepharose™ CL-2B has been used as resins in:
- gel permeation chromatography (GPC)
- gravity chromatography
- streptavidin affinity chromatography
- size exclusion chromatography
Legal Information
Sepharose is a trademark of Cytiva
1 of 1
This Item | |||
|---|---|---|---|
| form suspension | form beads | form - | form suspension |
| bead diameter 60-200 μm | bead diameter 40-165 μm | bead diameter - | bead diameter 40-165 μm |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level 200 |
| pore size 100,000-20,000,000 fractionation range (Dextrans), 70,000-40,000,000 fractionation range (Globular proteins) | pore size 30,000-5,000,000 fractionation range (Dextrans), 60,000-20,000,000 fractionation range (Globular proteins) | pore size - | pore size 10,000-1,000,000 fractionation range (Dextrans), 10,000-4,000,000 fractionation range (Globular proteins) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C |
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
102.2 °F - closed cup
flash_point_c
39 °C - closed cup
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H J Grimek et al.
Biology of reproduction, 30(2), 397-409 (1984-03-01)
Acetone precipitates of bovine follicular fluid from small (3-5 mm) and large (10-20 mm) follicles were fractionated by nondissociative procedures involving chromatography on Sephacryl S-300, DEAE cellulose, and Sepharose CL-2B. Most of the proteoglycan material eluted with a relatively low
Mouse liver mitochondria isolation, size fractionation, and real-time MOMP measurement
Renault TT, et al.
Bio-protocol, 6(15) (2016)
G. W. Cheng et al.
Plant physiology, 111(2), 447-457 (1996-06-01)
The locule tissue of tomato (Lycopersicon esculentum, Mill.) undergoes extensive liquefaction during ripening. In this study, the solubility, molecular mass, and glycosyl composition of locule pectic and alkali-soluble polysaccharides were examined with the aim of identifying features contributing to the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CL2B300-500ML | 04061833531860 |
| CL2B300-100ML | 04061833531853 |
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Is it possible to autoclave this resin and if so is there an existing protocol?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Bonjour, Pourriez vous m'indiquer à quoi correspond la taille des pore de a sepharose CL2B (CL2B300-500mL) en nm? Cordialement
1 answer-
Unfortunately, the pore size has not been determined. The average pore size for cross-linked agarose is approximately 25 nm. However, the actual pore size is not uniform.
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