You can use MTT to assess the intactness of isolated mitochondria. For this purpose, the CT01-5 Sigma-Aldrich MTT Reagent A is suitable. Additionally, the following publication may be of interest as a protocol reference: PLoS One. 2015 Jul 29;10(7):e0134139. This publication discusses the prevention of cisplatin-induced acute kidney injury and can provide valuable insights.
CS0390
Mitochondria Staining Kit
1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)
Synonym(s):
JC-1 dye
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $608.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352207
$608.00
Check Cart for Availability
Quality Segment
usage
kit sufficient for 200 tests (of 1 mL cell suspensions), kit sufficient for 40 tests (of 5 mL cell suspensions)
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
flow cytometry: suitable, protein staining: suitable
fluorescence
λex 490 nm; λem 530 nm (green) (JC-1 monomers), λex 525 nm; λem 590 nm (red) (JC-1 aggregates)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
General description
In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.
Application
The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.
Features and Benefits
Kit offers:
- A fast, simple, and convenient method for staining and assaying mitochondria intactness
- Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential
- Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation
- The shift in fluorescence is clearly detectable in both green and red channels
- Suitable for adherent cells as well as cells in suspension
- Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells
- The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry
1 of 1
This Item | |||
|---|---|---|---|
| description 1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions) | description The MitoLight Apoptosis Detection Kit for flow cytometry utilizes a lipophilic cation, termed as MitoLight, as a mitochondrial activity marker. | description A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria. | description Live cell imaging mitochondrial dye that stains the membrane of mitochondria used to detect cell viability, metabolic activity and overall cell health. |
| technique(s) protein staining: suitable, flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) - | technique(s) cell based assay: suitable |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level - |
| storage temp. 2-8°C | storage temp. - | storage temp. −20°C | storage temp. -10 to -25°C |
| application(s) cell analysis | application(s) - | application(s) - | application(s) - |
| fluorescence λex 490 nm; λem 530 nm (green) (JC-1 monomers), λex 525 nm; λem 590 nm (red) (JC-1 aggregates) | fluorescence - | fluorescence - | fluorescence - |
| shipped in wet ice | shipped in dry ice | shipped in ambient | shipped in ambient |
Kit Components Only
Product No.
Description
- DMSO 1 mL
- JC Staining Buffer 5X 120 mL
- JC-1 1 mg
- Valinomycin Ready Made .1 mL
Storage Class
10 - Combustible liquids
wgk
WGK 3
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