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D0672

φX174 DNA Marker Hae III Digest

for DNA electrophoresis

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Pack SizeSKUAvailabilityPrice
20 μg
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$327.00
0.1 mg
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$662.00

About This Item

NACRES:
NA.25
UNSPSC Code:
41105335
Form:
liquid
Storage temp.:
−20°C

$327.00


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Quality Level

form

liquid

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Preparation Note

φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

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Quality Level

100, 200

Quality Level

100, 200

Quality Level

100, 200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

usage

100 uses (0.1 mg size), 1000 uses (1 mg size), 20 uses (20 μg size), 500 uses (0.5 mg size)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)


Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Articles

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.





Global Trade Item Number

SKUGTIN
D0672-20UG04061835572465
D0672-.1MG04061833557969

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