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DMEM - low glucose

Name: DMEM - low glucose
Brand: SIGMA
Price: 68.2 CAD

With sodium pyruvate, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable fore cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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Pack Size	SKU	Availability	Price
100 mL		Please contact Customer Service for Availability	$68.20

About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217342

$68.20

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Properties

Product Name

Dulbecco′s Modified Eagle′s Medium - low glucose, 10 ×, With 1000 mg/L glucose (1x), without L-glutamine, sodium bicarbonate, and folic acid, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO₃: no
sodium pyruvate: yes
phenol red: yes
glucose: low
L-glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

Dulbecco′s Modified Eagle′s Medium (DME) is a modification of basal medium Eagle (BME) that contains a 4-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose.

Application

Dulbecco′s Modified Eagle′s Medium-low glucose has been used:

during organoid embedding for neutralizing thawed collagen I
to culture melanoma cancer cell lines
to store the cancer tissue samples
as a differentiation culture medium for C2C12 cells

Preparation Note

Supplement with 3.7 g/L sodium bicarbonate, 0.584 g/L L-glutamine, 0.004 g/L folic acid, at 1×.

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This Item	D5921	D5523	D5546
Sigma-Aldrich D2429 Dulbecco′s Modified Eagle′s Medium - low glucose	Sigma-Aldrich D5921 Dulbecco′s Modified Eagle′s Medium - low glucose	Sigma-Aldrich D5523 Dulbecco′s Modified Eagle′s Medium - low glucose	Sigma-Aldrich D5546 Dulbecco′s Modified Eagle′s Medium - low glucose
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
sterility sterile-filtered	sterility sterile-filtered	sterility -	sterility sterile-filtered
form liquid	form liquid	form powder	form liquid
components NaHCO₃: no sodium pyruvate: yes phenol red: yes glucose: low L-glutamine: no HEPES: no	components phenol red: no sodium pyruvate: no glucose: low HEPES: no L-glutamine: no NaHCO₃: yes	components L-glutamine: yes NaHCO₃: no sodium pyruvate: yes phenol red: yes HEPES: no glucose: low	components NaHCO₃: yes phenol red: yes glucose: low HEPES: no sodium pyruvate: yes L-glutamine: no
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C