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D2944

Anti-D1 Dopamine Receptor antibody,Rat monoclonal

clone 1-1-F11 s.E6, purified from hybridoma cell culture

Synonym(s):

Anti-DRD1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.25
MDL number:
MFCD00282304
Conjugate:
unconjugated
Clone:
1-1-F11 s.E6, monoclonal
Application:
ICC, IHC, WB
Citations:
60
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biological source

rat

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1-1-F11 s.E6, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat, monkey, human

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using D1 dopamine receptor transfected HEK-293T cells, immunohistochemistry: suitable, western blot: suitable

isotype

IgG2a

UniProt accession no.

P21728

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DRD1(1812)
rat ... Drd1a(24316)

General description

D1 Dopamine Receptor encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases.
Monoclonal Anti-D1 Dopamine Receptor (rat IgG2a isotype) is derived from the rat hybridoma 1-1-F11 S.E6 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor. Dopamine receptors belong to the family of seven transmembrane domain G protein coupled receptors (GPCRs). Dopamine receptors are mainly localized in the striatum, limbic system, the brain cortex and the infundibulum. The D1-like family consists of the D1 and D5 receptors.

Immunogen

recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-D1 Dopamine Receptor antibody produced in rat has been used in:
  • immunohistochemistry
  • immunoblotting
  • immunocytochemistry
  • proximity ligation assay
  • co-immunoprecipitation
  • western blotting

Biochem/physiol Actions

D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. D1 receptors are expressed on airway smooth muscle (ASM) and regulate smooth muscle force via cAMP activation of protein kinase A. It is a potential target for therapeutic relaxation of ASM.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

Sold under exclusive license from Emory University

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-D1 Dopamine Receptor antibody,Rat monoclonal clone 1-1-F11 s.E6, purified from hybridoma cell culture

Sigma-Aldrich

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Sigma-Aldrich

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Sigma-Aldrich

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Monoclonal Anti-AGO2 antibody produced in rat ~1.5 mg/mL, clone 11A9, purified immunoglobulin

Sigma-Aldrich

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Monoclonal Anti-AGO2 antibody produced in rat

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

Gene Information

human ... DRD1(1812)
rat ... Drd1a(24316)

Gene Information

human ... CDH1(999)
mouse ... Cdh1(12550)

Gene Information

mouse ... Cdh1(12550)

Gene Information

human ... AGO2(27161)
rat ... Ago2(59117)

biological source

rat

biological source

rat

biological source

rat

biological source

rat

species reactivity

rat, monkey, human

species reactivity

bovine, human, canine, mouse

species reactivity

dog, mouse

species reactivity

monkey, rat, human, mouse, bovine

clone

1-1-F11 s.E6, monoclonal

clone

DECMA-1, monoclonal

clone

DECMA-1, monoclonal

clone

11A9, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000519193
0000519193
0000430833
0000430833
0000352383

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D1R/GluN1 complexes in the striatum integrate dopamine and glutamate signalling to control synaptic plasticity and cocaine-induced responses
Cahill E, et al.
Molecular Psychiatry, 19(12), 1295-1295 (2014)
Impact of early consumption of high-fat diet on the mesolimbic dopaminergic system
Naneix F, et al.
eNeuro, 4(3) (2017)
Satoshi Goto et al.
Frontiers in neuroanatomy, 9, 22-22 (2015-03-19)
Immunohistochemistry (IHC) is a valuable method for identifying discrete neurochemical molecules by the interaction of target antigens with validated antibodies tagged with a visible label (e.g., peroxidase). We have developed an immunostaining method that is highly sensitive in detection of
View All Related Papers

Global Trade Item Number

SKUGTIN
D2944-100UL04061833562741

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