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D4138

Deoxyribonuclease II from porcine spleen

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Synonym(e):

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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Ihnen/SKUVerfügbarkeitPreis
20000 units

Ab HEUTE lieferbarvonSchnelldorf

59,40 €
80000 units

Ab HEUTE lieferbarvonSchnelldorf

179,00 €

Über diesen Artikel

CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-801-8
MDL number:
EG-Nummer:
Specific activity:
2,000-6,000 Kunitz units/mg protein (biuret)

59,40 €


Ab HEUTE lieferbarDetails


Technischer Dienst
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type

Type IV

Quality Segment

form

lyophilized powder

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

mol wt

45 kDa

composition

Protein, 60-80%

input

general use sample(s)

foreign activity

RNase ≤0.05%

storage temp.

−20°C

Application

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase.[1] It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).[2]
Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Biochem/physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions.[3] It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.[4]

Physical form

Contains sodium chloride

Other Notes

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25°C; [Mg2+] = 0.83 mM

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Dieser Artikel
D8764D4513D4527
description

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

description

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

description

Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

description

Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

specific activity

≥1,000 units/mg protein

specific activity

≥2,000 units/mg protein

specific activity

≥2,000 units/mg protein

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

45 kDa

mol wt

38 kDa

mol wt

~31 kDa

mol wt

~31 kDa

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

foreign activity

RNase ≤0.05%

foreign activity

RNase ≤0.1%

foreign activity

Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%

foreign activity

Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%


Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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