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D4138

Deoxyribonuclease II from porcine spleen

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Sinonimo/i:

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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A voi/SKUDisponibilitàPrezzo
20000 units
Per conoscere la disponibilità, visualizza il carrello
CHF 56.30
80000 units
Per conoscere la disponibilità, visualizza il carrello
CHF 186.00
CHF 158.10

Informazioni su questo articolo

Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-801-8
MDL number:
Numero CE:
Specific activity:
2,000-6,000 Kunitz units/mg protein (biuret)

CHF 56.30


Per conoscere la disponibilità, visualizza il carrello

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type

Type IV

Quality Segment

form

lyophilized powder

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

mol wt

45 kDa

composition

Protein, 60-80%

input

general use null

foreign activity

RNase ≤0.05%

storage temp.

−20°C

Application

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase.[1] It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).[2]
Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Biochem/physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions.[3] It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.[4]

Physical form

Contains sodium chloride

Other Notes

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25°C; [Mg2+] = 0.83 mM

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Questo articolo
D8764D4513D4527
specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

specific activity

≥1,000 units/mg protein

specific activity

≥2,000 units/mg protein

specific activity

≥2,000 units/mg protein

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

45 kDa

mol wt

38 kDa

mol wt

~31 kDa

mol wt

~31 kDa

foreign activity

RNase ≤0.05%

foreign activity

RNase ≤0.1%

foreign activity

Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%

foreign activity

Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%

composition

Protein, 60-80%

composition

-

composition

Protein, ≥80%

composition

Protein, ≥80%


Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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