Key Documents

View All Documentation

Deoxyribonuclease II from porcine spleen

Name: Deoxyribonuclease II from porcine spleen
Brand: SIGMA

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Synonym(s):

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

Select a Size

Change View

Size/SKU	Availability	Price

About This Item

CAS Number:

9025-64-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-801-8

MDL number:

MFCD00130919

EC Number:

3.1.22.1 (BRENDA, IUBMB)

Specific activity:

2,000-6,000 Kunitz units/mg protein (biuret)

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

type

Type IV

Quality Segment

200

form

lyophilized powder

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

mol wt

45 kDa

composition

Protein, 60-80%

input

general use sample(s)

foreign activity

RNase ≤0.05%

storage temp.

−20°C

Description

Application

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase.[1] It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).[2]

Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Biochem/physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions.[3] It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.[4]

Physical form

Contains sodium chloride

Other Notes

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25°C; [Mg²⁺] = 0.83 mM

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	D8764	D4513	D4527
Sigma-Aldrich D4138 Deoxyribonuclease II from porcine spleen	Sigma-Aldrich D8764 Deoxyribonuclease II from bovine spleen	Sigma-Aldrich D4513 Deoxyribonuclease I from bovine pancreas	Sigma-Aldrich D4527 Deoxyribonuclease I from bovine pancreas
description Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)	description Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein	description Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein	description Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein
specific activity 2,000-6,000 Kunitz units/mg protein (biuret)	specific activity ≥1,000 units/mg protein	specific activity ≥2,000 units/mg protein	specific activity ≥2,000 units/mg protein
form lyophilized powder	form essentially salt-free, lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 45 kDa	mol wt 38 kDa	mol wt ~31 kDa	mol wt ~31 kDa
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 300
foreign activity RNase ≤0.05%	foreign activity RNase ≤0.1%	foreign activity Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%	foreign activity Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.