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D4943

Dipeptidyl Peptidase IV human

recombinant, expressed in baculovirus infected Sf9 cells, pkg of ≥1.0 units/vial, ≥10 units/mg protein

Synonym(s):

CD26, DPPIV, Dipeptidyl aminopeptidase IV, Glycoprotein GP110

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Size/SKUAvailabilityPrice
1 vial
Please contact Customer Service for Availability
₪3,607.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.4.14.5 (BRENDA, IUBMB)
MDL number:
MFCD00166456
Specific activity:
≥10 units/mg protein
Recombinant:
expressed in baculovirus infected Sf9 cells

₪3,607.00

Please contact Customer Service for Availability
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recombinant

expressed in baculovirus infected Sf9 cells

Quality Segment

200

form

solution

specific activity

≥10 units/mg protein

mol wt

105 kDa

packaging

pkg of ≥1.0 units/vial

UniProt accession no.

P27487

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... DPP4(1803)

General description

C-terminal histidine-tagged. Soluble form (residues 29-766) MW 105 kDa

Application

Human dipeptidyl peptidase IV has been used to study interactive hemodynamic effects of its inhibition and angiotensin-converting enzyme inhibition in humans. Human dipeptidyl peptidase IV has also been used in a study that informed the understanding of Hymenoptera venom allergies.
The enzyme from Sigma has been used to study the LC-MS (liquid chromatography-mass spectrometry) based assay method for DPP-IV inhibitor screening and substrate discovery.

Biochem/physiol Actions

DPPIV has a post-proline dipeptidyl aminopeptidase activity that hydrolyzes N-terminal dipeptides from the unsubstituted N-terminus of peptides with the sequence of X-Pro-Z and X-Ala-Z. The optimum pH is found to be 7.4-8.7. DPPIV is involved in the regulation of several important physiological processes such as immune functions, inflammation, CNS, endocrine functions, bone marrow mobilization, cancer growth, cell adhesion, glucose hemostasis and sepsis/severe infection.
DPPIV has a post-proline dipeptidyl aminopeptidase activity that hydrolyzes N-terminal dipeptides from the unsubstituted N-terminus of peptides with the sequence of X-Pro-Z and X-Ala-Z. Where X is a nonspecific residue at the N terminus and Z cannot be proline or hydroxyproline.
Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. There is an 88% sequence homology between the human and porcine kidney enzymes. Both exist as homodimers with a subunit molecular weight of ~30 kDa. The high mannose 100 kDa DPPIV precursor is processed in the Golgi to yield a 124 kDa heavily N-and O-linked mature glycoprotein. It is then sorted to the apical membrane through the concerted action of both N- and O-linked glycans and its association with lipid microdomains. The porcine enzyme contains 18.3% carbohydrates, which the glycan composition is 0.9% fucose, 3.4% mannose, 5.1% galactose, 8.2% glucosamine, and 0.7% sialic acid. DPPIV is highly expressed on endothelial cells, epithelial cells, and lymphocytes. It is also present in plasma in its soluble form.

Physical form

Supplied as a solution in 10 mM Tris-HCl, pH 7.6, 200 mM NaCl, 1 mM EDTA and 10% glycerol.

Other Notes

One unit will produce 1.0 μmole of p-nitroaniline from Gly-L-Pro p-nitroanilide per min in 100 mM Tris-HCl at pH 7.6 at 37 °C.
View more information on Dipeptidyl Peptidase IV at www.sigma-aldrich.com/enzymeexplorer.

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1 of 1

This Item
D3446MABD45WH0001803M3
Dipeptidyl Peptidase IV human recombinant, expressed in baculovirus infected Sf9 cells, pkg of ≥1.0 units/vial, ≥10 units/mg protein

Sigma-Aldrich

D4943

Dipeptidyl Peptidase IV human

Dipeptidyl Peptidase IV human recombinant, expressed in Sf9 cells

Sigma-Aldrich

D3446

Dipeptidyl Peptidase IV human

Anti-DDPIV/CD26 Antibody, clone 9F1.2 clone 9F1.2, from mouse

Sigma-Aldrich

MABD45

Anti-DDPIV/CD26 Antibody, clone 9F1.2

Monoclonal Anti-DPP4 antibody produced in mouse clone 1C5, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

WH0001803M3

Monoclonal Anti-DPP4 antibody produced in mouse

description

recombinant, expressed in baculovirus infected Sf9 cells, pkg of ≥1.0 units/vial, ≥10 units/mg protein

description

recombinant, expressed in Sf9 cells

description

clone 9F1.2, from mouse

description

clone 1C5, purified immunoglobulin, buffered aqueous solution

specific activity

≥10 units/mg protein

specific activity

≥4,000 units/μg protein

specific activity

-

specific activity

-

Gene Information

human ... DPP4(1803)

Gene Information

human ... DPP4(1803)

Gene Information

human ... DPP4(1803)

Gene Information

human ... DPP4(1803)

form

solution

form

solution

form

-

form

buffered aqueous solution

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in Sf9 cells

recombinant

-

recombinant

-

storage temp.

−20°C

storage temp.

−70°C

storage temp.

-

storage temp.

−20°C

mol wt

105 kDa

mol wt

124 kDa

mol wt

-

mol wt

-

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

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Questions

1–2 of 2 Questions  

  1. 1. The product information was 1 unit/ vial. How microliter or how much 1 vial ? 2. if we will make 0,005 u/ml enzyme, how?

    1 answer

    1. The volume in each vial can be calculated from information provided in the lot specific certificate of analysis found here:
      https://www.sigmaaldrich.com/product/sigma/d4943#product-documentation

      The specification is that each vial will be provided at a concentration of ≥10ug protein/mL, and that the activity will be ≥ 10units per mg protein. The calculations to make a solution of 0.005U/mL will be dependent on the specific activity of the lot.

      Helpful?

  2. 이 효소 제품은 Gly-Pro p-nitroanilide만을 분해할 수 있나요? D3446의 Specification Sheet에는 Ala-Pro-AMC를 분해한다고 나와 있는데, D4943은 Gly-Pro p-nitroanilide만을 분해할 수 있고 D3446은 Ala-Pro-AMC만을 분해할 수 있는건지 알고싶습니다.

    1 answer

    1. As described above, DPPIV has a post-proline dipeptidyl aminopeptidase activity that hydrolyzes N-terminal dipeptides from the unsubstituted N-terminus of peptides with the sequence of X-Pro-Z and X-Ala-Z, where X is a nonspecific residue at the N-terminus and Z cannot be proline or hydroxyproline. pNA (p-nitroanilide) and AMC (7-amino-4-methylcoumarin) are substrate conjugates that are used for the quantitative analysis of enzyme activities by chromogenic or fluorogenic detection, respectively.

      Helpful?

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