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D5671

DMEM - high glucose

With sodium Bicarbonate, without ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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Pack SizeSKUAvailabilityPrice
500 mL
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€33.00
1 L
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€62.70
6 x 500 mL
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€178.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

€33.00

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Product Name

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
glucose: high
NaHCO3: yes
sodium pyruvate: no
phenol red: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

General description

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Supplement with 0.584 g/L L-glutamine.

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This Item
D5796D6546D6429
Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

D5671

Dulbecco′s Modified Eagle′s Medium - high glucose

Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D5796

Dulbecco′s Modified Eagle′s Medium - high glucose

Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D6546

Dulbecco′s Modified Eagle′s Medium - high glucose

Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D6429

Dulbecco′s Modified Eagle′s Medium - high glucose

form

liquid

form

liquid

form

liquid

form

liquid

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
glucose: high
NaHCO3: yes
sodium pyruvate: no
phenol red: yes
HEPES: no

components

NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

components

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0550
RNBM0069
RNBL8843
RNBL7075
RNBL6563

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Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

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Protocole du test au MTT pour la mesure de la viabilité, de la prolifération et de la toxicité cellulaires. Instructions de préparation du réactif au MTT et exemples d'applications.

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Global Trade Item Number

SKUGTIN
D5671-1L04061833586587
D5671-20L04061837340437
D5671-24X500ML04061833586594
D5671-10L04061837340420
D5671-6X1L04061833586617
D5671-6X500ML04061833586624
D5671-500ML04061833586600

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