This is 10 mM each and can be found under Description-> General Description: The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water.
D7295
Deoxynucleotide Mix, 10 mM
Molecular Biology Reagent
Synonym(s):
Deoxynucleotide Mix, 10 mM, 10mM dNTP mix, dNTP mix, dNTPs
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.2 mL | Check Cart for Availability | $60.30 | |
| 0.5 mL | Check Cart for Availability | $123.00 | |
| 20 x 0.2 mL | Check Cart for Availability | $789.00 | |
| 5 mL | Check Cart for Availability | $792.00 |
About This Item
NACRES:
NA.52
UNSPSC Code:
41106305
Assay:
≥99%
Biological source:
synthetic (organic)
Form:
liquid
Solubility:
H2O: soluble at 20 °C
Storage temp.:
−20°C
biological source
synthetic (organic)
Quality Level
assay
≥99%
form
liquid
concentration
10 mM
color
colorless
solubility
H2O: soluble at 20 °C
application(s)
agriculture
foreign activity
DNase, RNase, none detected
shipped in
dry ice
storage temp.
−20°C
General description
Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.
Application
dNTP Mix has been used:
- in the PCR amplification of genomic DNA isolated from insect, fungi,[1] virus, human,[2]
- in reverse transcription of total RNA to cDNA.[3][3]
- as a component of the DNA amplification mixture for polymerase chain reaction (PCR)[1][4]
- routine and long PCR
- manual and automated DNA sequencing
- cDNA synthesis[5] and labeling reactions
Features and Benefits
- Purity of each dNTP: Minimum 99%
- Conveniently formulated; 1 μL is used per 50 μL PCR
- Equimolar amounts of each dNTP means less pipetting
- Minimize risk of contamination in PCR
- UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions
1 of 1
This Item | |||
|---|---|---|---|
| biological source synthetic (organic) | biological source - | biological source - | biological source - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| form liquid | form liquid | form liquid | form liquid |
| assay ≥99% | assay ≥99% | assay - | assay - |
| concentration 10 mM | concentration 10 mM | concentration 100 mM | concentration 100 mM |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.
Articles
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
Related Content
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
Shangmei Cao et al.
Journal of ethnopharmacology, 244, 112104-112104 (2019-08-09)
ShenYanXiaoBai granules is a traditional Chinese herbal medicine, It is used widely for the treatment of proteinuria caused by various kidney diseases. This study investigated the mechanism of Shenyan Xiaobai Granule in the treatment of nephritis proteinuria. 100 male wistar
Abril Sánchez-Botet et al.
Scientific reports, 8(1), 11797-11797 (2018-08-09)
Colorectal cancer (CRC) is one of the most common cancers worldwide, with 8-10% of these tumours presenting a BRAF (V600E) mutation. Cyclins are known oncogenes deregulated in many cancers, but the role of the new subfamily of atypical cyclins remains
Unal Egeli et al.
Cancer investigation, 24(5), 484-491 (2006-08-31)
BRCA1 and BRCA2 gene mutations in patients with breast and/or ovarian cancer have been not characterized in the Turkish population until now. A total of 87 female subjects from two sets of families (38 families total) provided blood samples from
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D7295-.5ML | 04061838104946 |
| D7295-5ML | 04061838104953 |
| D7295-.25ML | 04061838056863 |
| D7295-.2ML | 04061838104939 |
| D7295-20X.2ML | 04061838087355 |
-
Is this 10 mM each, or 10 mM total? This info should be clearly stated on the label and on your site.
1 answer-
Helpful?
-
-
How much Product D7295, Deoxynucleotide Mix, is needed for a PCR reaction?
1 answer-
One μl is sufficient for a standard 50 μl PCR reaction.
Helpful?
-
-
Does Product No. D7295, Deoxynucleotide Mix contain dTTP or TTP?
1 answer-
The product contains Thymidine 5´-triphosphate sodium salt. Thymidine has a deoxy sugar, so it is a deoxythymidine.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What are the components to Product D7295, Deoxynucleotide Mix?
1 answer-
The D7295 contains 10mM of each dATP, dCTP, dGTP and TTP in molecular biology grade water. This is for a total concentration of 40 mM.
Helpful?
-