Accéder au contenu
MilliporeSigma

Principaux documents

Voir toute la documentation

Passer à

D8276

DNA Polymerase I, Klenow Fragment from Escherichia coli

buffered aqueous glycerol solution

Se connecter pour consulter les tarifs organisationnels et contractuels.

Sélectionner une taille de conditionnement

Changer de vue
À vous/RéférenceDisponibilitéPrix
50 units
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
208,00 $
100 units
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
333,00 $

A propos de cet article

Numéro CAS:
9012-90-2
UNSPSC Code:
12352204
NACRES:
NA.53
MDL number:
MFCD00283091
Concentration:
~3,000 units/mL

208,00 $

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
​
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aider

grade

Molecular Biology

Quality Segment

200

form

buffered aqueous glycerol solution

mol wt

103 kDa

concentration

~3,000 units/mL

UniProt accession no.

P00582

foreign activity

Endonuclease, none detected

shipped in

wet ice

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... polA(948356)

General description

DNA polymerase I yields two fragments (small and large) upon protease digestion. The large fragment (Klenow fragment) loses the 5′ exonuclease activity that is present in the intact holoenzyme. However, it retains both the polymerase 5′→3′ activity and the 3′→5′ exonuclease activity of the native enzyme.

Application

Suitable for:
  • DNA sequencing by the Sanger dideoxy method
  • Synthesis of the complementary strand of cDNA
  • Filling in 5′-overhangs in double stranded DNA to form blunt ends
  • Mutagenesis of DNA with second strand synthesis using oligonucleotides
  • Labeling DNA by the random primer method

Preparation Note

The enzyme solution may be diluted with 50 mM Tris-HCl, pH 7.5, 100 mM ammonium sulfate, 10 mM 2-mercaptoethanol, and 1 mg/ml bovine serum albumin.

Analysis Note

The activity is assayed in a reaction mixture containing 50 mM potassium phosphate (pH 7.5), 3 mM MgCl2, 1 mM 2-mercaptoethanol, 32.5 μM 32P-dATP, 32.5 μM dTTP, 62.5 μg/ml poly(dA-dT) and 0.01-1 unit enzyme.

Other Notes

DNA Polymerase I is supplied as a solution in 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 5 mM dithiothretol, and 50% glycerol (v/v) .
One unit converts 10 nanomoles of deoxyribonucleoside triphosphates into acid insoluble material in 30 min. at 37 °C.

Comparer avec des articles similaires

Voir la comparaison complète

Montrer les différences

1 of 1

Cet article
D9380D0690F3174
DNA Polymerase I, Klenow Fragment from Escherichia coli buffered aqueous glycerol solution

Sigma-Aldrich

D8276

DNA Polymerase I, Klenow Fragment from Escherichia coli

DNA Polymerase I from Escherichia coli lysogenic for NM 964 buffered aqueous glycerol solution

Sigma-Aldrich

D9380

DNA Polymerase I from Escherichia coli lysogenic for NM 964

DNA Gyrase from Escherichia coli aqueous glycerol solution

Sigma-Aldrich

D0690

DNA Gyrase from Escherichia coli

Fpg Protein from Escherichia coli ≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis

Sigma-Aldrich

F3174

Fpg Protein from Escherichia coli

description

buffered aqueous glycerol solution

description

buffered aqueous glycerol solution

description

aqueous glycerol solution

description

≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis

grade

Molecular Biology

grade

Molecular Biology

grade

-

grade

-

Gene Information

Escherichia coli K12 ... polA(948356)

Gene Information

Escherichia coli K12 ... polA(948356)

Gene Information

Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Gene Information

Escherichia coli CFT073 ... mutM(1038243)
Escherichia coli K12 ... mutM(946765)

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

aqueous glycerol solution

form

buffered aqueous glycerol solution

concentration

~3,000 units/mL

concentration

5,000-15,000 units/mL

concentration

≥2 unit/μL

concentration

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

Si vous avez besoin d'assistance, veuillez contacter Service Clients

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Questions

Reviews

No rating value

Active Filters