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D9380

DNA Polymerase I from Escherichia coli lysogenic for NM 964

buffered aqueous glycerol solution

Synonym(s):

Kornberg Polymerase

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500 units
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$281.00
2000 units
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$917.00

About This Item

CAS Number:
9012-90-2
UNSPSC Code:
12352204
NACRES:
NA.53
EC Number:
2.7.7.7 (BRENDA, IUBMB)
MDL number:
MFCD00130924
Concentration:
5,000-15,000 units/mL

$281.00

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grade

Molecular Biology

Quality Level

200

form

buffered aqueous glycerol solution

mol wt

109 kDa

concentration

5,000-15,000 units/mL

UniProt accession no.

P00582

foreign activity

Endonuclease, none detected

shipped in

wet ice

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... polA(948356)

General description

DNA polymerase I (holoenzyme) has 5′→3′ and 3′→5′ exonuclease activities in addition to its synthetic activity. This bifunctional activity enables the enzyme to use nicks or gaps in double stranded DNA as starting points for DNA synthesis. The 5′→3′ exonuclease activity degrades the DNA strand complementary to the template strand beginning at the nick. DNA synthesis begins at the 3′-end of the nick and produces a new strand of DNA complementary to the template. The net result is the movement of the polymerase along the template strand (nick translation) until the DNA complementary to the template (from the site of the original nick to the 5′-end of the template strand) is replaced.

Application

Suitable for:
  • Highly specific DNA probes by nick translation
  • In vitro synthesis of complementary cDNA strand
  • In vitro synthesis of DNA
  • Produce blunt ends from 5′ and 3′ overhangs
DNA Polymerase I from Escherichia coli has been used to study the effects of the anti-tumor drug cis-diaminedichloroplatinum (II) on the enzyme activity.

Other Notes

DNase Polymerase I is supplied in a solution of 50% glycerol containing 100 mM potassium phosphate buffer (pH 6.5), and 1 mM dithiothreitol.
One unit converts 10 nanomoles of deoxyribonucleoside triphosphates into acid insoluble material in 30 min at 37 °C.

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This Item
D8276F3174D0690
DNA Polymerase I from Escherichia coli lysogenic for NM 964 buffered aqueous glycerol solution

Sigma-Aldrich

D9380

DNA Polymerase I from Escherichia coli lysogenic for NM 964

DNA Polymerase I, Klenow Fragment from Escherichia coli buffered aqueous glycerol solution

Sigma-Aldrich

D8276

DNA Polymerase I, Klenow Fragment from Escherichia coli

Fpg Protein from Escherichia coli ≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis

Sigma-Aldrich

F3174

Fpg Protein from Escherichia coli

DNA Gyrase from Escherichia coli aqueous glycerol solution

Sigma-Aldrich

D0690

DNA Gyrase from Escherichia coli

grade

Molecular Biology

grade

Molecular Biology

grade

-

grade

-

Gene Information

Escherichia coli K12 ... polA(948356)

Gene Information

Escherichia coli K12 ... polA(948356)

Gene Information

Escherichia coli CFT073 ... mutM(1038243)
Escherichia coli K12 ... mutM(946765)

Gene Information

Escherichia coli K12 ... gyrA(946614), gyrB(948211)

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

aqueous glycerol solution

concentration

5,000-15,000 units/mL

concentration

~3,000 units/mL

concentration

-

concentration

≥2 unit/μL

UniProt accession no.

P00582

UniProt accession no.

P00582

UniProt accession no.

P05523

UniProt accession no.

P0AES4, P0AES6

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000461188
0000461188
0000440717
0000440717
SLCC8483

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Related Content

Lehman, I.R., et al.
The Enzymes, 14A, 16-38 (1981)
Inhibition of Escherichia coli DNA polymerase-I by the anti-cancer drug cis-diaminedichloroplatinum(II): what roles do polymerases play in cis-platin-induced cytotoxicity?
Rebecca K
Febs Letters (1999)
Micrococcus luteus deoxyribonucleic acid polymerase. Studies of the enzymic reaction and properties of the deoxyribonucleic acid product.
S J Harwood et al.
The Journal of biological chemistry, 245(21), 5614-5624 (1970-11-10)
View All Related Papers

Global Trade Item Number

SKUGTIN
D9380-500UN04061833590652
D9380-2KU04061833651629

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