D9893
pBR322 Plasmid DNA from E. coli RRI
lyophilized powder
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| Size/SKU | Availability | Price |
|---|---|---|
1 unit | Check Cart for Availability | $529.00 |
5 units | Check Cart for Availability | $2,120.00 |
About This Item
CAS Number:
UNSPSC Code:
12352200
eCl@ss:
32160414
EC Number:
297-227-2
NACRES:
NA.52
MDL number:
$529.00
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biological source
Escherichia coli
Quality Segment
grade
Molecular Biology
form
lyophilized powder
mol wt
2.9 MDa, 4363 bp
storage temp.
2-8°C
General description
Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.
The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).
The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).
Application
pBR322 Plasmid DNA from E. coli RRI has been used as a low molecular weight DNA to test the enzymatic activity of partially purified component from Perna viridis (Linn.) It has also been used for investigating its effect on the calcium carbonate particle morphology by scanning electron microscopy studies
Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication.
Biochem/physiol Actions
Unique Sites: Within the gene for ampicillin resistance: Pst I, Pvu I, Sca I; Within the gene for tetracycline resistance: BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, Xma III; other unique sites: Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, Tth111 I.
One of the most commonly used cloning vectors, pBR322, confers resistance to ampicillin and tetracycline. The DNA sequence of the entire plasmid has been published.
This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.
Other Notes
Accession number: J01749
Accession number: J01749
DNA is provided as a powder, lyophilized from a solution of 1 mM Tris-HCl (pH 7.5) with 1mM NaCl and 1mM EDTA.
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This Item | |||
|---|---|---|---|
| description lyophilized powder | description buffered aqueous solution | description pUC18, buffered aqueous solution | description pUC19, buffered aqueous solution |
| form lyophilized powder | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| mol wt 2.9 MDa, 4363 bp | mol wt 4363 bp, 2.9 MDa | mol wt - | mol wt - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| biological source Escherichia coli | biological source - | biological source Escherichia coli (RRI) | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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