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Carboxylesterase 2 human

Name: Carboxylesterase 2 human
Brand: SIGMA
Price: 94300 JPY

recombinant, expressed in baculovirus infected BTI insect cells

Synonym(s):

Carboxylesterase 2 human, CES2, CES2A1

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Pack Size	SKU	Availability	Price
1 vial		Check Cart for Availability	¥94,300

About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.1.1.1 (BRENDA, IUBMB)

Specific activity:

≥500 units/mg protein

Recombinant:

expressed in baculovirus infected BTI insect cells

¥94,300

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Properties

recombinant

expressed in baculovirus infected BTI insect cells

Quality Level

200

form

liquid

specific activity

≥500 units/mg protein

mol wt

60 kDa

weight

(0.5 ml)

shipped in

dry ice

storage temp.

−70°C

Description

General description

Carboxylesterase 2 human gene is mapped to human chromosome 16q22.1.[1]

Carboxylesterase belongs to the α/β hydrolase fold family and is involved in drug metabolism and activation. It is present in colon, liver, small intestine, heart, brain and testis. The hydrolase activity of small intestine is attributed to carboxylesterase 2 human.[2][3] It has two glycosylation sites at 103 and 267 residues with molecular mass 60kDa.[4]

Application

Carboxylesterase 2 human has been used:

in the in vitro enzyme-based inhibitor screening assay[5]
in substrate selectivity assay with emission ratiometric two-photon probe (SE1)[6]
as a reference standard in the enzyme assay with various 4-nitrophenyl and 1-naphthyl based substrates[7]

Carboxylesterase 2 may play a key role in biotransformation of a variety of ester containing drugs and prodrugs.[3]

Delivers high catalytic activity, ideal for robust high-throughput screening assays including drug-drug interaction studies, and pharmacokinetic studies for evaluating pro-drugs and non-CYP pathways of elimination.

Biochem/physiol Actions

Carboxylesterase 2 human identifies the site containing a large alcohol and small acyl group.[8] It shows less transesterification activity due to presence of conformational interference in active site.[3]

Member of a serine esterase family that hydrolyze ester and amide bonds. Carboxylesterase 2 is an endoplasmic reticulum-bound hydrolase that plays a critical role in xenobiotic detoxification and activation for ester-containing therapeutics. Carboxylesterase 2 is also involved in the detoxification of drugs such as heroin and cocaine. This enzyme is thought to play a role in lipid metabolism.

Other Notes

One unit will produce 1 nanomole of 4-nitrophenol from 4-nitrophenyl acetate per minute at pH 7.4 at 37 deg C.

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1 of 1

This Item	E0287	C4749	E0162
Sigma-Aldrich E0412 Carboxylesterase 2 human	Sigma-Aldrich E0287 Carboxylesterase 1 isoform b human	Sigma-Aldrich C4749 Carboxylesterase 2 human	Sigma-Aldrich E0162 Carboxylesterase 1 isoform c human
specific activity ≥500 units/mg protein	specific activity ≥500 units/mg protein	specific activity ≥1.0 EU/μg, 30,000 pmol/min-μg protein	specific activity -
form liquid	form liquid	form solution	form liquid
recombinant expressed in baculovirus infected BTI insect cells	recombinant expressed in baculovirus infected BTI insect cells	recombinant expressed in mouse NSO cells	recombinant expressed in baculovirus infected BTI insect cells
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
storage temp. −70°C	storage temp. −70°C	storage temp. −70°C	storage temp. −70°C
mol wt 60 kDa	mol wt -	mol wt predicted mol wt ~60 kDa	mol wt -

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act Listed

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E0412-VAR: + E0412-1VL: + E0412-1VL-PW: + E0412-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number

0000480625

0000464685

0000431524

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The mammalian carboxylesterases: from molecules to functions.

T Satoh et al.

Annual review of pharmacology and toxicology, 38, 257-288 (1998-05-23)

Multiple carboxylesterases (EC 3.1.1.1) play an important role in the hydrolytic biotransformation of a vast number of structurally diverse drugs. These enzymes are major determinants of the pharmacokinetic behavior of most therapeutic agents containing ester or amide bonds. Carboxylesterase activity

Human liver carboxylesterase. Purification and molecular properties.

W Junge et al.

Archives of biochemistry and biophysics, 165(2), 749-763 (1974-12-01)

A carboxylesterase-selective ratiometric fluorescent two-photon probe and its application to hepatocytes and liver tissues.

Sang Jun Park et al.

Chemical science, 7(6), 3703-3709 (2016-06-01)

Carboxylesterases (CEs) are widely distributed enzymes in the human body that catalyze hydrolysis of various endogenous and exogenous substrates. They are directly linked to hepatic drug metabolisms and steatosis, and their regulations are important issues in pharmacological and clinical applications.

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Global Trade Item Number

SKU	GTIN
E0412-1VL	04061826590829

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