The Enzymatic Assay for Pullulanase is described in full detail here:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/517/754/pullulanase.pdf?srsltid=AfmBOoowPYNfQjzj1eyi5czeqN9kE7fYBqx-72QH5gfC3fOBMAbjm36v
E2412
Pullulanase microbial
Synonym(s):
Pullulanase microbial, Promozyme® D2
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 mL | Available to ship TODAYfromMILWAUKEE | $138.00 | |
| 250 mL | Estimated to ship onMay 19, 2026fromMILWAUKEE | $340.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥1000 NPUN/g
Recombinant:
expressed in Bacillus subtilis
$138.00
Available to ship TODAYDetails
recombinant
expressed in Bacillus subtilis
Quality Level
form
aqueous solution
specific activity
≥1000 NPUN/g
storage temp.
2-8°C
Application
Pullulanase is a glucanase that degrades pullulan. It is commonly used for starch-debranching as well as to study polysaccharide utilization[1].
Biochem/physiol Actions
Type I pullulanases specifically hydrolyse α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Legal Information
A product of Novozymes Corp.
Novozym is a registered trademark of Novozymes A/S
Promozyme is a trademark of Novozymes Corp.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥1000 NPUN/g | specific activity ≥100 units/mg protein, ≥70 units/mg dry wt. | specific activity - | specific activity - |
| form aqueous solution | form powder | form liquid | form buffered aqueous glycerol solution |
| recombinant expressed in Bacillus subtilis | recombinant - | recombinant - | recombinant - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
Nasima Chorfa et al.
Polymers, 14(10) (2022-05-29)
In this work, a valorization of the starch stemming from downgraded potatoes was approached through the preparation of starch nanoparticles using different physical methods, namely liquid and supercritical carbon dioxide, high energy ball milling (HEBM), and ultrasonication on the one
K A Smith et al.
Journal of bacteriology, 171(4), 2116-2123 (1989-04-01)
We have cloned a pullulanase gene from Bacteroides thetaiotaomicron. The pullulanase expressed from this clone in Escherichia coli was cell associated and soluble and had a molecular mass of 72 kilodaltons by gel filtration. Maxicell analysis of proteins coded by
Christopher C Ibenegbu et al.
Microbial cell factories, 21(1), 251-251 (2022-11-30)
The starch in waste bread (WB) from industrial sandwich production was directly converted to ethanol by an amylolytic, ethanologenic thermophile (Parageobacillus thermoglucosidasius strain TM333) under 5 different simultaneous saccharification and fermentation (SSF) regimes. Crude α-amylase from TM333 was used alone
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E2412-250ML | 04061833602614 |
| E2412-50ML | 04061833658321 |
-
What is the optimum pH and media solution for Pullulanase activity?
1 answer-
Helpful?
-
-
What is the meaning of NPUN/g of pullulanase enzyme E2412?
1 answer-
The activity is expressed as NPUN/g, which refers to New Pullulanase Unit Novozymes (NPUN) per milligram of protein. The international unit correspondence is described as follows: 1 NPUN = 0.35 PUN (Pullulanase Unit Novozymes), where 1 PUN is defined as the amount of enzyme that, under standard conditions, releases 1 ?mol of glucose per minute.
Helpful?
-
-
I was wondering the proportion of type 1 and type2 in pullulanase?
1 answer-
Product Code E2412 contains both Pullulanase 1 and 2, but the exact ratio is considered proprietary.
Helpful?
-
-
May I know how to make 10units/mL sample from the bottle?
1 answer-
The activity in the batch-specific Certificate of Analysis is expressed as NPUN/g. The international unit correspondence is described as follows: 1 NPUN = 0.35 PUN, where 1 PUN is defined as the amount of enzyme that releases 1 μmol of glucose per minute under standard conditions.
The Certificate of Analysis also reports the density (g/mL) of the product. To calculate the activity in units per milliliter (UPUN/mL), multiply the activity (NPUN/g) by the density (g/mL). Further dilution can then be performed to achieve the desired final concentration.
Helpful?
-
-
Could you let me know if there are other substances included in this product for stabilization purposes, aside from the pullulanase enzyme? If so, could you specify what those additives are?
1 answer-
The composition of this product is considered proprietary by the supplier, and the components have not been provided. For inquiries about the composition, please contact the supplier directly.
Helpful?
-
-
I was wondering if product E2412-50ML is a type 1 or type 2 pullulanase (degrades alpha1,6 linkages only or both alpha1,4 and 1,6?)
1 answer-
E2412 contains both Type 1 and Type 2 Pullulanases.
Helpful?
-
-
What is the molecular weight of this Pullulanase enzyme? And is there any glucose in the enzyme solution?
1 answer-
The molecular weight has not been determined, however, a search of the literature indicates that the general molecular weight of Pullulanase, CAS 9075-68-7, ranges from 70 - 115 kDa. This is a product of Novozymes Corp. The manufacturer considers the formulation of this product proprietary but has noted that this material is pure Oligo-1,6-Glucosidase.
Helpful?
-