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Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

Name: Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse
Brand: SIGMA
Price: 748 USD
Availability: InStock

clone 29.1, ascites fluid

Synonym(s):

Anti-ERBB, Anti-ERBB1, Anti-ERRP, Anti-HER1, Anti-NISBD2, Anti-PIG61, Anti-mENA

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Pack Size	SKU	Availability	Price
0.2 mL		Available to ship TODAYfromMILWAUKEE	$748.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162282

Conjugate:

unconjugated

Clone:

29.1, monoclonal

Application:

ELISA (i), WB

Citations:

$748.00

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Properties

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

29.1, monoclonal

contains

15 mM sodium azide

species reactivity

pig, human, rat, mouse, canine

should not react with

rabbit

technique(s)

indirect ELISA: 1:10,000 using extract of the A431 cell line, western blot: 1:3,000 using extract of the A431 cell line

isotype

IgG1

UniProt accession no.

P00533

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)

Description

General description

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. A close similarity has been found between the sequence of the avian erythroblastic leukemia viral oncogene homolog (v-erb-B) and the cytoplasmic and transmembrane part of the EGF-R (truncated EGF-R).

Immunogen

human carcinoma cell line A431, which expresses high levels of EGF receptors.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

May be used to purify EGF receptor, for structural and functional studies of the receptor and for investigation of cell transformation versus oncogenesis.

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in

enzyme linked immunosorbent assay (ELISA)
immunoprecipitation
immunoaffinity
immunohistochemistry[1]
western blotting[2]

Biochem/physiol Actions

Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding.

The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (Neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	E3138	SAB1405746	E1157
Sigma-Aldrich E2760 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse	Sigma-Aldrich E3138 Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse	Sigma-Aldrich SAB1405746 Anti-EGFR antibody produced in mouse	Sigma-Aldrich E1157 Anti-Epidermal Growth Factor Receptor/Erb B1 antibody produced in goat
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
clone 29.1, monoclonal	clone F4, monoclonal	clone polyclonal	clone polyclonal
antibody form ascites fluid	antibody form ascites fluid	antibody form purified immunoglobulin	antibody form affinity isolated antibody
biological source mouse	biological source mouse	biological source mouse	biological source goat
Gene Information human ... EGFR(1956) mouse ... Egfr(13649) rat ... Egfr(24329)	Gene Information human ... EGFR(1956)	Gene Information human ... EGFR(1956)	Gene Information human ... EGFR(1956)
species reactivity pig, human, rat, mouse, canine	species reactivity human	species reactivity human	species reactivity human

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000512401

0000448246

099M4828V

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Global Trade Item Number

SKU	GTIN
E2760-.2ML	04061838105059
E2760-50UL	04061836275648
E2760-100UL	04061835503834

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