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E2761

ExtrAvidin®−FITC

buffered aqueous solution

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UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00163600
Form:
buffered aqueous solution

160,00 €

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conjugate

FITC conjugate

Quality Segment

200

form

buffered aqueous solution

extent of labeling

3-6 mol fluorochrome per mol protein

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues, indirect immunofluorescence: 1:200

shipped in

dry ice

storage temp.

−20°C

General description

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.[1] It consists of four high affinity binding sites for biotin. ExtrAvidin is prepared from egg white avidin. It is a modified form of affinity purified avidin and combines high specific activity of avidin with the low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii.[2] ExtrAvidin has been conjugated to fluorescein isothiocyanate (FITC) and is used for various techniques.

Application

ExtrAvidin®-FITC is suitable for the following applications:
  • immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 20μg/mL using human tissues.[3][4]
  • Immunofluorescence[5][6]†
  • competitive binding assay[7]

Physical form

Solution in 0.01 M phosphate buffered saline, containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide

Preparation Note

Affinity purified protein

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Cet article
E2636A2050E4011
ExtrAvidin®−FITC buffered aqueous solution

Sigma-Aldrich

E2761

ExtrAvidin®−FITC

ExtrAvidin® –Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

E2636

ExtrAvidin® –Alkaline Phosphatase

Avidin–FITC from egg white buffered aqueous solution

Sigma-Aldrich

A2050

Avidin–FITC from egg white

ExtrAvidin®−R-Phycoerythrin buffered aqueous solution

Sigma-Aldrich

E4011

ExtrAvidin®−R-Phycoerythrin

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues, indirect immunofluorescence: 1:200

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL, indirect immunofluorescence: 1:200

technique(s)

flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

dry ice

shipped in

ambient

shipped in

dry ice

shipped in

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

FITC conjugate

conjugate

alkaline phosphatase conjugate

conjugate

FITC conjugate

conjugate

phycoerythrin (R-PE) conjugate

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

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