Yes, casein or nonfat dry milk may be used as a blocking solution. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/370/767/e2886dat-ms.pdf
E2886
ExtrAvidin®−Peroxidase
buffered aqueous solution
Synonym(s):
ExtrAvidin HRP
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.2 mL | Check Cart for Availability | $137.00 | |
| 1 mL | Check Cart for Availability | $438.00 |
About This Item
Form:
buffered aqueous solution
conjugate
peroxidase conjugate
Quality Level
form
buffered aqueous solution
technique(s)
direct ELISA: 1:2,000, dot blot: 1:4,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
shipped in
wet ice
storage temp.
2-8°C
Application
Biochem/physiol Actions
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.[15] It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii.[16] ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.
The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Preparation Note
Affinity purified protein
Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| technique(s) direct ELISA: 1:2,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, dot blot: 1:4,000 | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 | technique(s) dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000 | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1,000, western blot: 1:5,000-1:20,000 |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in ambient | shipped in wet ice |
| conjugate peroxidase conjugate | conjugate CY3 conjugate | conjugate alkaline phosphatase conjugate | conjugate unconjugated |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| E2886-5X1ML | 04061835807581 |
| E2886-.2ML | 04061833602997 |
| E2886-1ML | 04061838446039 |
| E2886-.15ML | 04061835807574 |
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Biotinylated protein을 detection하기 위하여 ExtrAvidin-peroxidase를 ELISA에 사용하고자하는데, well blocking solution으로 casein이나 Nonfat Dry Milk 등을 사용해도 괜찮을까요?
1 answer-
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