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Earle′s Balanced Salts

Name: Earle′s Balanced Salts
Brand: SIGMA

With sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

EBSS

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217343

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Properties

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: no
NaHCO₃: 2.2 g/L
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

room temp

Description

General description

Earle′s balanced salts solution (EBSS) is a saline solution with physiological pH that stimulates autophagy. It augments Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) by upregulating cluster of differentiation (CD86), human leukocyte antigen-DR isotype (HLA-DR), and interleukin (IL-6).[1]

Application

Earle′s balanced salts has been used to incubate filter-grown monolayers of Caco-2 cells to induce starvation[2], along with HEPES buffer to detect NADPH oxidase activity in trypsinized cells.[3] It has also been used to treat CEM RES cells and perform amino acid depletion experiment.[4]

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

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This Item	E2888	E7510	E6267
Sigma-Aldrich E3024 Earle′s Balanced Salts	Sigma-Aldrich E2888 Earle′s Balanced Salts	Sigma-Aldrich E7510 Earle′s Balanced Salt Solution 10x	Sigma-Aldrich E6267 Earle′s Balanced Salts
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form liquid	form liquid
components phenol red: no NaHCO₃: 2.2 g/L glucose: 1.0 g/L (Dextro)	components phenol red: 0.011 g/L glucose: 1.0 g/L (Dextro) NaHCO₃: 2.2 g/L	components glucose: 10.0 g/L NaHCO₃: no phenol red: 0.11 g/L	components NaHCO₃: 2.2 g/L phenol red: 0.011 g/L glucose: 1.0 g/L (Dextro)
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. room temp	storage temp. room temp	storage temp. room temp	storage temp. room temp

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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