β-Estradiol 6-((O-carboxy-methyl)oxime: BSA, Product Number E5630, is soluble in PBS to 0.90-1.10 mg/mL, and this will yield a faintly hazy to hazy, colorless to faintly yellow solution. Accordingly, E5630-5MG is soluble in 4.5-5.6 mL of PBS. The actual batch-specific solubility is listed on the corresponding CoA. Please download the CoA from the “Documents” section of the PDP at the following link:
https://www.sigmaaldrich.com/product/sigma/e5630#product-documentation
E5630
β-Estradiol 6-(O-carboxymethyl)oxime: BSA
mitogen-activated protein kinase (MAPK) stimulator, lyophilized powder
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| Size/SKU | Availability | Price |
|---|---|---|
5 mg | Please contact Customer Service for Availability | $320.00 |
10 mg | Please contact Customer Service for Availability | $551.00 |
About This Item
Form:
lyophilized powder
Product Name
β-Estradiol 6-(O-carboxymethyl)oxime: BSA,
sterility
non-sterile
Quality Segment
form
lyophilized powder
extent of labeling
~30 mol steroid per mol BSA
solubility
phosphate buffer: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
shipped in
ambient
storage temp.
2-8°C
General description
Application
β-Estradiol 6-(O-carboxymethyl)oxime: BSA has been used:
- to test its effect on the N-methyl-D-aspartate receptor (NMDAR) currents in female dorsal root ganglion (DRG) neurons[2]
- to coat microplates for the detection of plasma 17β-Estradiol levels using indirect enzyme-linked immunosorbent assay (ELISA)[3]
- to test its effect on MutL homolog 1 in colorectal cancer lines[4]
Biochem/physiol Actions
1 of 1
This Item | |||
|---|---|---|---|
| form lyophilized powder | form lyophilized powder | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| solubility phosphate buffer: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow | solubility 0.1 M phosphate buffer: 0.98-1.02 mg/mL, clear, yellow to orange (pH: 7.5) | solubility ethanol: 19.60-20.40 mg/mL, clear, colorless to faintly yellow | solubility 0.1 M NaOH: 10 mg/mL, clear to slightly hazy, colorless to faintly yellow |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| sterility non-sterile | sterility - | sterility - | sterility non-sterile |
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Buongiorno, il datasheet incluso con il prodotto E56-30 non è chiaro. Potrei sapere il volume finale in cui sciogliere 5mg di E2/BSA e la relativa concentrazione?
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What is the solution stability of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
1 answer-
We have not performed solution stability studies, and have found no information on solution stability in the literature. We would therefore recommend using freshly prepared solutions.
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How is Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA, prepared?
1 answer-
Our method of preparation is considered proprietary information However, a method of preparation (not necessarily Sigma-Aldrich’s) has been described in J. Biol. Chem., 228, 713-727 (1957).
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What is the solubility of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
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We have solubilized the product in phosphate buffered saline at 1 mg/mL. Solutions may be somewhat hazy.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the shelf life of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
1 answer-
There is no assigned shelf life date for the product. The guarantee of quality is one year from the time customer receives the product for unopened bottles stored at 2-8°C.
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What is the molecular weight of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
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The Certificate of Analysis has lot specific information for the number of moles of steroid per mole of bovine serum albumin. From this data and the molecular weights of the components, an approximate molecular weight for the compound can be calculated. For example, if the moles of beta estradiol to BSA is 32, then the approximate molecular weight for the compound is 76.4 kDa (BSA has a molecular weight of approximately 66.4 kDa and 32 moles of estradiol is approximately 10 kDa more).
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